EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.35 | 2-Aminonicotinate | activates | Cutaneotrichosporon cutaneum | |
1.14.13.35 | N-Formyl anthranilate | activates | Cutaneotrichosporon cutaneum | |
1.14.13.35 | salicylate | activates | Cutaneotrichosporon cutaneum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.13.35 | 50000 | - |
2 * 50000, SDS-PAGE | Cutaneotrichosporon cutaneum |
1.14.13.35 | 95000 | - |
gel filtration | Cutaneotrichosporon cutaneum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.35 | Cutaneotrichosporon cutaneum | - |
yeast | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.35 | using ammonium sulfate precipitation, column chromatography on DE23-cellulose, phenyl-Sepharose and S-300 Sephacryl | Cutaneotrichosporon cutaneum |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.14.13.35 | anthranilate + NADPH + H+ + O2 = 2,3-dihydroxybenzoate + NADP+ + NH3 | mechanism proposed involving imine formation and hydrolysis during the reaction with the flavin peroxide formed from reduced enzyme flavin and molecular oxygen | Cutaneotrichosporon cutaneum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.13.35 | 4.4 | - |
- |
Cutaneotrichosporon cutaneum |
EC Number | Storage Stability | Organism |
---|---|---|
1.14.13.35 | -20°C, in the dark, Na+/K+ phosphate buffer, pH 7.4, 0.1 mM EDTA, stable for at least 6 months | Cutaneotrichosporon cutaneum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.35 | 2-hydrazinebenzoate + NADH + O2 | - |
Cutaneotrichosporon cutaneum | ? | - |
? | |
1.14.13.35 | 3-hydroxyanthranilate + NADPH + O2 | - |
Cutaneotrichosporon cutaneum | ? | - |
? | |
1.14.13.35 | 3-methylanthranilate + NADPH + O2 | - |
Cutaneotrichosporon cutaneum | ? + NADP+ + NH3 | - |
? | |
1.14.13.35 | anthranilate + NADPH + O2 | - |
Cutaneotrichosporon cutaneum | 2,3-dihydroxybenzoate + NADP+ + NH3 | 2,3-dihydroxybenzoate i.e. o-pyrocatechuate, oxygen atom at the 3-position of the product 2,3-dihydroxybenzoate originates from O2, that at the 2-position is derived from H2O | ? | |
1.14.13.35 | N,N-dimethylanthranilate + NADPH + O2 | - |
Cutaneotrichosporon cutaneum | ? | - |
? | |
1.14.13.35 | N-methylanthranilate + NADPH + O2 | - |
Cutaneotrichosporon cutaneum | 2,3-dihydroxybenzoate + NADP+ + NH3 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.13.35 | dimer | 2 * 50000, SDS-PAGE | Cutaneotrichosporon cutaneum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.13.35 | 25 | - |
assay at | Cutaneotrichosporon cutaneum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.35 | 7.7 | - |
- |
Cutaneotrichosporon cutaneum |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.35 | 5.5 | 9.8 | less than 1% of maximal activity at pH 5.5 and pH 9.8 | Cutaneotrichosporon cutaneum |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.35 | 5.9 | 9.1 | 4°C, stable for at least 3 days | Cutaneotrichosporon cutaneum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.35 | FAD | flavoprotein | Cutaneotrichosporon cutaneum | |
1.14.13.35 | FAD | 2 mol FAD per mol enzyme | Cutaneotrichosporon cutaneum | |
1.14.13.35 | NADPH | - |
Cutaneotrichosporon cutaneum |