Literature summary extracted from
Vancurova, I.; Flieger, M.; Volc, J.; Benes, M.J.; Novotna, J.; Neuzil, J.; Behal, V.
Partial purification and characterization of anhydrotetracycline oxygenase of Streptomyces aureofaciens (1987), J. Basic Microbiol., 27, 529-533.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
1.14.13.38 |
additional information |
addition of supernatant of Streptomyces aureofaciens or Streptomyces rimosus crude extract after boiling and centrifugation increases activity 7fold |
Kitasatospora aureofaciens |
|
General Stability
EC Number |
General Stability |
Organism |
---|
1.14.13.38 |
loss of activity during purification |
Kitasatospora aureofaciens |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
1.14.13.38 |
dithiobis-(2-nitrobenzoic acid) |
40% inhibition at 1 mM, irreversible |
Kitasatospora aureofaciens |
|
1.14.13.38 |
iodoacetamide |
14% inhibition at 1 mM, irreversible |
Kitasatospora aureofaciens |
|
1.14.13.38 |
N-ethylmaleimide |
complete inhibition at 0.1 M, irreversible |
Kitasatospora aureofaciens |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
1.14.13.38 |
cytoplasm |
- |
Kitasatospora aureofaciens |
5737 |
- |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.14.13.38 |
Fe2+ |
increase of activity |
Kitasatospora aureofaciens |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.14.13.38 |
57500 |
- |
2 * 57500, SDS-PAGE |
Kitasatospora aureofaciens |
1.14.13.38 |
115000 |
- |
gel filtration |
Kitasatospora aureofaciens |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
1.14.13.38 |
anhydrotetracycline + NADPH + O2 |
Kitasatospora aureofaciens |
tetracycline and chlortetracycline biosynthetic pathways |
12-dehydrotetracycline + NADP+ + H2O |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.14.13.38 |
Kitasatospora aureofaciens |
- |
50/137, UV-light induced mutant of strain 84/25 |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.14.13.38 |
- |
Kitasatospora aureofaciens |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
1.14.13.38 |
-25°C, crude extract stable for several weeks |
Kitasatospora aureofaciens |
1.14.13.38 |
4°C, crude extract stable for several days |
Kitasatospora aureofaciens |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.14.13.38 |
anhydrotetracycline + NADPH + O2 |
- |
Kitasatospora aureofaciens |
12-dehydrotetracycline + NADP+ + H2O |
- |
? |
|
1.14.13.38 |
anhydrotetracycline + NADPH + O2 |
tetracycline and chlortetracycline biosynthetic pathways |
Kitasatospora aureofaciens |
12-dehydrotetracycline + NADP+ + H2O |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.14.13.38 |
dimer |
2 * 57500, SDS-PAGE |
Kitasatospora aureofaciens |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
1.14.13.38 |
7.3 |
7.4 |
assay at |
Kitasatospora aureofaciens |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.14.13.38 |
ascorbate |
increase of activity |
Kitasatospora aureofaciens |
|
1.14.13.38 |
NADPH |
- |
Kitasatospora aureofaciens |
|