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Literature summary extracted from

  • Langkau, B.; Vock, P.; Massey, V.; Fuchs, G.; Ghisla, S.
    2-Aminobenzoyl-CoA monooxygenase/reductase. Evidence for two distinct loci catalyzing substrate monooxygenation and hydrogenation (1995), Eur. J. Biochem., 230, 676-685.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.40 0.026
-
 NADH
-
 Pseudomonas sp.
1.14.13.40 0.47
-
 N-ethylmaleimide
-
 Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.40 Pseudomonas sp.
-
-
-
1.14.13.40 Pseudomonas sp. KB740
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.40
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.40 additional information
-
 specific activity of isolated and reconstituted enzyme in different fractions after purification Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.14.13.40 -196°C stable Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.40 2-aminobenzoyl-CoA + NAD(P)H + O2 i.e. anthraniloyl-CoA Pseudomonas sp. 2-amino-5-oxocyclohex-1-enecarboxyl-CoA + H2O + NAD(P)+
-
 ?
1.14.13.40 2-aminobenzoyl-CoA + NAD(P)H + O2 i.e. anthraniloyl-CoA Pseudomonas sp. KB740 2-amino-5-oxocyclohex-1-enecarboxyl-CoA + H2O + NAD(P)+
-
 ?
1.14.13.40 2-hydroxybenzoyl-CoA + NAD(P)H + O2 i.e. salicoyl-CoA, weak substrate, is probably monooxygenated but not hydrogenated Pseudomonas sp. ?
-
 ?
1.14.13.40 2-hydroxybenzoyl-CoA + NAD(P)H + O2 i.e. salicoyl-CoA, weak substrate, is probably monooxygenated but not hydrogenated Pseudomonas sp. KB740 ?
-
 ?
1.14.13.40 anthraniloyl-CoA + NADH + H+ + O2
-
 Pseudomonas sp. 2-amino-5-oxocyclohex-1-enecarboxyl-CoA + H2O + NAD+
-
 r
1.14.13.40 anthraniloyl-CoA + NADH + H+ + O2
-
 Pseudomonas sp. KB740 2-amino-5-oxocyclohex-1-enecarboxyl-CoA + H2O + NAD+
-
 r
1.14.13.40 N-ethylmaleimide + NAD(P)H + H+
-
 Pseudomonas sp. N-ethylsuccinimide + NAD(P)+
-
 ?
1.14.13.40 N-ethylmaleimide + NAD(P)H + H+
-
 Pseudomonas sp. KB740 N-ethylsuccinimide + NAD(P)+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.40 dimer three dimeric forms: 1. alpha,alpha, 2. alpha',alpha', 3. alpha,alpha' Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.13.40
-
-
 50% loss of activity in 2 days when stored highly concentrated, in dilute solutions it loses 50% of activity in 2 min, NADH, FAD and 2-aminobenzoyl CoA enhance stability Pseudomonas sp.
1.14.13.40 25
-
 50% loss of activity in 2 days when stored highly concentrated Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.40 FAD the three dimeric forms alphaalpha, alpha'alpha' and alphaalpha' differ in their mode of binding FAD Pseudomonas sp.
1.14.13.40 NADH
-
 Pseudomonas sp.