EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.7 | dithiothreitol | 20% increase of activity at 1 mM | Cutaneotrichosporon cutaneum |
EC Number | General Stability | Organism |
---|---|---|
1.14.13.7 | chloride destabilizes | Cutaneotrichosporon cutaneum |
1.14.13.7 | phosphate stabilizes | Cutaneotrichosporon cutaneum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.7 | 1,10-phenanthroline | slight inhibition at 0.0005 to 0.001 mM | Cutaneotrichosporon cutaneum | |
1.14.13.7 | AgNO3 | 0.01 mM | Cutaneotrichosporon cutaneum | |
1.14.13.7 | ammonium sulfate | - |
Cutaneotrichosporon cutaneum | |
1.14.13.7 | ascorbate | 52% inhibition at 50 mM | Cutaneotrichosporon cutaneum | |
1.14.13.7 | Cl- | 90% inhibition at 0.1 M, irreversible | Cutaneotrichosporon cutaneum | |
1.14.13.7 | copper-chelating agents | not | Cutaneotrichosporon cutaneum | |
1.14.13.7 | CuSO4 | 0.01 mM | Cutaneotrichosporon cutaneum | |
1.14.13.7 | FeSO4 | 0.1 mM | Cutaneotrichosporon cutaneum | |
1.14.13.7 | Guanidinium chloride | 70-80% inhibition at 0.1 M | Cutaneotrichosporon cutaneum | |
1.14.13.7 | H2O2 | 71% inhibition at 0.1 M | Cutaneotrichosporon cutaneum | |
1.14.13.7 | HgCl2 | 0.01 mM | Cutaneotrichosporon cutaneum | |
1.14.13.7 | p-chloromercuribenzoate | inhibition is reversed by dithiothreitol | Cutaneotrichosporon cutaneum | |
1.14.13.7 | Peroxidase | - |
Cutaneotrichosporon cutaneum | |
1.14.13.7 | SDS | - |
Cutaneotrichosporon cutaneum | |
1.14.13.7 | Sodium borohydride | - |
Cutaneotrichosporon cutaneum | |
1.14.13.7 | Sodium dithionite | - |
Cutaneotrichosporon cutaneum | |
1.14.13.7 | Triton X-100 | - |
Cutaneotrichosporon cutaneum | |
1.14.13.7 | Urea | 70-80% inhibition at 2 M | Cutaneotrichosporon cutaneum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.7 | 0.018 | - |
phenol | - |
Cutaneotrichosporon cutaneum | |
1.14.13.7 | 0.053 | - |
O2 | - |
Cutaneotrichosporon cutaneum | |
1.14.13.7 | 0.071 | - |
NADPH | - |
Cutaneotrichosporon cutaneum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.7 | additional information | does not contain heme, non-heme iron or copper | Cutaneotrichosporon cutaneum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.13.7 | 148000 | - |
gel filtration | Cutaneotrichosporon cutaneum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.7 | Cutaneotrichosporon cutaneum | - |
yeast | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.7 | homogeneity | Cutaneotrichosporon cutaneum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.13.7 | 8.3 | - |
- |
Cutaneotrichosporon cutaneum |
EC Number | Storage Stability | Organism |
---|---|---|
1.14.13.7 | -20°C, 4-6 weeks stable | Cutaneotrichosporon cutaneum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.7 | 2-aminophenol + O2 + NADPH | - |
Cutaneotrichosporon cutaneum | ? | - |
? | |
1.14.13.7 | 2-chlorophenol + O2 + NADPH | - |
Cutaneotrichosporon cutaneum | ? | - |
? | |
1.14.13.7 | 2-fluorophenol + O2 + NADPH | - |
Cutaneotrichosporon cutaneum | ? | - |
? | |
1.14.13.7 | 3-aminophenol + O2 + NADPH | - |
Cutaneotrichosporon cutaneum | ? | - |
? | |
1.14.13.7 | 3-chlorophenol + O2 + NADPH | - |
Cutaneotrichosporon cutaneum | ? | - |
? | |
1.14.13.7 | 3-fluorophenol + O2 + NADPH | - |
Cutaneotrichosporon cutaneum | ? | - |
? | |
1.14.13.7 | 4-aminophenol + O2 + NADPH | - |
Cutaneotrichosporon cutaneum | ? | - |
? | |
1.14.13.7 | 4-chlorophenol + O2 + NADPH | - |
Cutaneotrichosporon cutaneum | ? | - |
? | |
1.14.13.7 | 4-fluorophenol + O2 + NADPH | - |
Cutaneotrichosporon cutaneum | ? | - |
? | |
1.14.13.7 | additional information | broad specificity, reaction results in the formation of the corresponding o-diols | Cutaneotrichosporon cutaneum | ? | - |
? | |
1.14.13.7 | phenol + NADPH + O2 | - |
Cutaneotrichosporon cutaneum | catechol + NADP+ + H2O | - |
? | |
1.14.13.7 | quinol + O2 + NADPH | - |
Cutaneotrichosporon cutaneum | 1,2,4-trihydroxybenzene + NADP+ + H2O | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.7 | 7.2 | 7.6 | in phosphate buffer | Cutaneotrichosporon cutaneum |
1.14.13.7 | 8.2 | - |
in Tris-Cl buffer | Cutaneotrichosporon cutaneum |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.7 | 5 | - |
stable for at least 7 days | Cutaneotrichosporon cutaneum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.7 | FAD | flavoprotein | Cutaneotrichosporon cutaneum | |
1.14.13.7 | FAD | 1 mol FAD per mol of enzyme | Cutaneotrichosporon cutaneum | |
1.14.13.7 | NADPH | - |
Cutaneotrichosporon cutaneum |