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Literature summary extracted from

  • Neujahr, H.Y.; Gaal, A.
    Phenol hydroxylase from yeast. Purification and properties of the enzyme from Trichosporon cutaneum (1973), Eur. J. Biochem., 35, 386-400.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.13.7 dithiothreitol 20% increase of activity at 1 mM Cutaneotrichosporon cutaneum

General Stability

EC Number General Stability Organism
1.14.13.7 chloride destabilizes Cutaneotrichosporon cutaneum
1.14.13.7 phosphate stabilizes Cutaneotrichosporon cutaneum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.7 1,10-phenanthroline slight inhibition at 0.0005 to 0.001 mM Cutaneotrichosporon cutaneum
1.14.13.7 AgNO3 0.01 mM Cutaneotrichosporon cutaneum
1.14.13.7 ammonium sulfate
-
Cutaneotrichosporon cutaneum
1.14.13.7 ascorbate 52% inhibition at 50 mM Cutaneotrichosporon cutaneum
1.14.13.7 Cl- 90% inhibition at 0.1 M, irreversible Cutaneotrichosporon cutaneum
1.14.13.7 copper-chelating agents not Cutaneotrichosporon cutaneum
1.14.13.7 CuSO4 0.01 mM Cutaneotrichosporon cutaneum
1.14.13.7 FeSO4 0.1 mM Cutaneotrichosporon cutaneum
1.14.13.7 Guanidinium chloride 70-80% inhibition at 0.1 M Cutaneotrichosporon cutaneum
1.14.13.7 H2O2 71% inhibition at 0.1 M Cutaneotrichosporon cutaneum
1.14.13.7 HgCl2 0.01 mM Cutaneotrichosporon cutaneum
1.14.13.7 p-chloromercuribenzoate inhibition is reversed by dithiothreitol Cutaneotrichosporon cutaneum
1.14.13.7 Peroxidase
-
Cutaneotrichosporon cutaneum
1.14.13.7 SDS
-
Cutaneotrichosporon cutaneum
1.14.13.7 Sodium borohydride
-
Cutaneotrichosporon cutaneum
1.14.13.7 Sodium dithionite
-
Cutaneotrichosporon cutaneum
1.14.13.7 Triton X-100
-
Cutaneotrichosporon cutaneum
1.14.13.7 Urea 70-80% inhibition at 2 M Cutaneotrichosporon cutaneum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.7 0.018
-
phenol
-
Cutaneotrichosporon cutaneum
1.14.13.7 0.053
-
O2
-
Cutaneotrichosporon cutaneum
1.14.13.7 0.071
-
NADPH
-
Cutaneotrichosporon cutaneum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.7 additional information does not contain heme, non-heme iron or copper Cutaneotrichosporon cutaneum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.7 148000
-
gel filtration Cutaneotrichosporon cutaneum

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.7 Cutaneotrichosporon cutaneum
-
yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.7 homogeneity Cutaneotrichosporon cutaneum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.7 8.3
-
-
Cutaneotrichosporon cutaneum

Storage Stability

EC Number Storage Stability Organism
1.14.13.7 -20°C, 4-6 weeks stable Cutaneotrichosporon cutaneum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.7 2-aminophenol + O2 + NADPH
-
Cutaneotrichosporon cutaneum ?
-
?
1.14.13.7 2-chlorophenol + O2 + NADPH
-
Cutaneotrichosporon cutaneum ?
-
?
1.14.13.7 2-fluorophenol + O2 + NADPH
-
Cutaneotrichosporon cutaneum ?
-
?
1.14.13.7 3-aminophenol + O2 + NADPH
-
Cutaneotrichosporon cutaneum ?
-
?
1.14.13.7 3-chlorophenol + O2 + NADPH
-
Cutaneotrichosporon cutaneum ?
-
?
1.14.13.7 3-fluorophenol + O2 + NADPH
-
Cutaneotrichosporon cutaneum ?
-
?
1.14.13.7 4-aminophenol + O2 + NADPH
-
Cutaneotrichosporon cutaneum ?
-
?
1.14.13.7 4-chlorophenol + O2 + NADPH
-
Cutaneotrichosporon cutaneum ?
-
?
1.14.13.7 4-fluorophenol + O2 + NADPH
-
Cutaneotrichosporon cutaneum ?
-
?
1.14.13.7 additional information broad specificity, reaction results in the formation of the corresponding o-diols Cutaneotrichosporon cutaneum ?
-
?
1.14.13.7 phenol + NADPH + O2
-
Cutaneotrichosporon cutaneum catechol + NADP+ + H2O
-
?
1.14.13.7 quinol + O2 + NADPH
-
Cutaneotrichosporon cutaneum 1,2,4-trihydroxybenzene + NADP+ + H2O
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.7 7.2 7.6 in phosphate buffer Cutaneotrichosporon cutaneum
1.14.13.7 8.2
-
in Tris-Cl buffer Cutaneotrichosporon cutaneum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.13.7 5
-
stable for at least 7 days Cutaneotrichosporon cutaneum

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.7 FAD flavoprotein Cutaneotrichosporon cutaneum
1.14.13.7 FAD 1 mol FAD per mol of enzyme Cutaneotrichosporon cutaneum
1.14.13.7 NADPH
-
Cutaneotrichosporon cutaneum