Any feedback?
Literature summary extracted from
- Diketocamphane enantiomer-specific 'Baeyer-Villiger' monooxygenases from camphor-grown Pseudomonas putida ATCC 17453 (1993), J. Gen. Microbiol., 139, 797-805.
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.14.108 | cytoplasm | NADH dehydrogenase component forming a loose complex with the oxygenating component | Pseudomonas putida | 5737 | - |
| 1.14.14.155 | cytoplasm | NADH dehydrogenase component forming a loosely complex with the oxygenating component | Pseudomonas putida | 5737 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.108 | additional information | - |
enzyme consists of 2 components: 1 oxygenating and 1 NADH dehydrogenase | Pseudomonas putida |
| 1.14.14.108 | additional information | - |
enzyme components form a very loose complex | Pseudomonas putida |
| 1.14.14.108 | 44000 | - |
about, oxygenating component, native PAGE | Pseudomonas putida |
| 1.14.14.155 | additional information | - |
enzyme consists of 2 components: 1 oxygenating and 1 NADH dehydrogenase | Pseudomonas putida |
| 1.14.14.155 | additional information | - |
enzyme components form a very loose complex | Pseudomonas putida |
| 1.14.14.155 | 40300 | - |
oxygenating component, native PAGE | Pseudomonas putida |
| 1.14.14.155 | 76000 | - |
both components, ultracentrifugal analysis | Pseudomonas putida |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.108 | Pseudomonas putida | - |
- |
- |
| 1.14.14.108 | Pseudomonas putida | - |
inducible by (+)-camphor | - |
| 1.14.14.108 | Pseudomonas putida C1 / ATCC 17453 | - |
- |
- |
| 1.14.14.155 | Pseudomonas putida | - |
inducible by (-)-camphor | - |
| 1.14.14.155 | Pseudomonas putida | - |
strain ATCC 17453 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.14.108 | both components | Pseudomonas putida |
| 1.14.14.155 | oxygenating component | Pseudomonas putida |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.108 | additional information | - |
no enzyme assay possible in crude extract due to competition with other enzymes for NADH | Pseudomonas putida |
| 1.14.14.108 | 0.335 | - |
substrate (+)-camphor | Pseudomonas putida |
| 1.14.14.155 | 0.244 | - |
substrate (-)-camphor | Pseudomonas putida |
| 1.14.14.155 | 300 | - |
purified enzyme component NADH dehydrogenase | Pseudomonas putida |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.108 | (+)-bornane-2,5-dione + FMNH2 + O2 | i.e. 2,5-diketocamphane | Pseudomonas putida | 3,4,4-trimethyl-5-carboxy-methyl-DELTA2-cyclopentenone + FMN + H2O | - |
? |  |
| 1.14.14.108 | (+)-bornane-2,5-dione + FMNH2 + O2 | i.e. 2,5-diketocamphane | Pseudomonas putida C1 / ATCC 17453 | 3,4,4-trimethyl-5-carboxy-methyl-DELTA2-cyclopentenone + FMN + H2O | - |
? | |
| 1.14.14.108 | (+)-bornanone + FMNH2 + O2 | (+)-camphor | Pseudomonas putida | 1,2-campholide + FMN + H2O | - |
? | |
| 1.14.14.108 | (+)-bornanone + FMNH2 + O2 | (+)-camphor | Pseudomonas putida C1 / ATCC 17453 | 1,2-campholide + FMN + H2O | - |
? | |
| 1.14.14.108 | additional information | enzyme is associated with a NADH oxidase | Pseudomonas putida | ? | - |
? | |
| 1.14.14.108 | additional information | rubredoxin not mentioned | Pseudomonas putida | ? | - |
? | |
| 1.14.14.108 | additional information | no activity with (-)-camphor and other ketocamphanes that do not possess a keto group at position 2 | Pseudomonas putida | ? | - |
? | |
| 1.14.14.108 | additional information | enzyme is associated with a NADH oxidase | Pseudomonas putida C1 / ATCC 17453 | ? | - |
? | |
| 1.14.14.108 | additional information | rubredoxin not mentioned | Pseudomonas putida C1 / ATCC 17453 | ? | - |
? | |
| 1.14.14.108 | additional information | no activity with (-)-camphor and other ketocamphanes that do not possess a keto group at position 2 | Pseudomonas putida C1 / ATCC 17453 | ? | - |
? | |
| 1.14.14.155 | (-)-bornanone + FMNH2 + O2 | i.e. (-)-camphor | Pseudomonas putida | ? | - |
? | |
| 1.14.14.155 | additional information | enzyme is associated with a NADH oxidase | Pseudomonas putida | ? | - |
? | |
| 1.14.14.155 | additional information | rubredoxin not mentioned | Pseudomonas putida | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.14.108 | More | enzyme components form a very loose complex | Pseudomonas putida |
| 1.14.14.108 | More | enzyme consists of 2 components: 1 oxygenating and 1 NADH dehydrogenase | Pseudomonas putida |
| 1.14.14.155 | monomer | 1 * 40300, oxygenating component, SDS-PAGE | Pseudomonas putida |
| 1.14.14.155 | More | enzyme consists of 2 components: 1 oxygenating and 1 NADH dehydrogenase | Pseudomonas putida |
| 1.14.14.155 | More | enzyme components from a very loose complex | Pseudomonas putida |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.108 | 2,5-diketocamphane 1,2-monooxygenase | - |
Pseudomonas putida |
| 1.14.14.108 | 3,6-diketocamphane 1,6-monooxygenase | different enzyme with similar biochemical properties, immunological cross-reactivity | Pseudomonas putida |
| 1.14.14.155 | 3,6-diketocamphane 1,6-monooxygenase | different enzyme with similar biochemical properties, immunological cross-reactivity | Pseudomonas putida |
| 1.14.14.155 | 3,6-diketocamphane 1,6-monooxygenase | isoform, which is probably a different enzyme | Pseudomonas putida |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.108 | FMN | binds reversibly | Pseudomonas putida | |
| 1.14.14.155 | FMN | binds reversibly | Pseudomonas putida | |
| 1.14.14.155 | additional information | absolute specificity | Pseudomonas putida | |
| 1.14.14.155 | additional information | interaction of inducible NADH dehydrogenase component via bound FMN with oxygenating component | Pseudomonas putida | |
| 1.14.14.155 | additional information | FAD is ineffective | Pseudomonas putida | |
| 1.14.14.155 | additional information | purified NADH dehydrogenase component from 2,5-diketocamphane-1,2-monooxygenase can be used as electron donor | Pseudomonas putida |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
