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Literature summary extracted from

  • Abita, J.P.; Blandin-Savoja, F.; Rey, F.
    Phenylalanine 4-monooxygenase from human liver (1987), Methods Enzymol., 142, 27-35.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.16.1 cAMP-dependent protein kinase
-
Homo sapiens
1.14.16.1 cAMP-dependent protein kinase
-
Rattus norvegicus
1.14.16.1 lysolecithin
-
Homo sapiens
1.14.16.1 lysolecithin
-
Rattus norvegicus
1.14.16.1 additional information relatively low activity with tetrahydrobiopterin can be selectively increased by limited proteolysis Homo sapiens
1.14.16.1 additional information relatively low activity with tetrahydrobiopterin can be selectively increased by limited proteolysis Rattus norvegicus
1.14.16.1 additional information enzyme activity is not stimulated by phosphorylation Homo sapiens
1.14.16.1 Phospholipids activate Homo sapiens
1.14.16.1 Phospholipids activate Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.16.1 0.0026
-
tetrahydrobiopterin
-
Homo sapiens
1.14.16.1 0.033
-
6,7-dimethyltetrahydropterin
-
Homo sapiens
1.14.16.1 0.05
-
phenylalanine cofactor tetrahydrobiopterin Homo sapiens
1.14.16.1 0.55
-
phenylalanine cofactor dimethyltetrahydropterin Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.16.1 54000
-
2 * 54000, fetal liver enzyme, SDS-PAGE Homo sapiens
1.14.16.1 107000
-
fetal liver enzyme, sucrose density gradient centrifugation Homo sapiens
1.14.16.1 165000
-
gel filtration Homo sapiens
1.14.16.1 275000
-
-
Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.1 Homo sapiens
-
-
-
1.14.16.1 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.14.16.1 side-chain modification
-
Homo sapiens
1.14.16.1 side-chain modification enzyme contains 0.3 mol of phosphate per mol of enzyme subunit Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.16.1 Phenyl-Sepharose, DEAE-Sepharose Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.16.1 liver
-
Homo sapiens
-
1.14.16.1 liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.16.1 0.106
-
-
Homo sapiens

Storage Stability

EC Number Storage Stability Organism
1.14.16.1 -80°C, enzyme concentration 1 mg/ml, several months, no loss of activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.1 L-phenylalanine + 6,7-dimethyltetrahydrobiopterin
-
Homo sapiens L-tyrosine + 6,7-dimethyl-4a-hydroxy-tetrahydrobiopterin
-
r
1.14.16.1 L-phenylalanine + tetrahydrobiopterin + O2 relatively low activity with tetrahydrobiopterin can be selectively increased by limited proteolysis Homo sapiens L-tyrosine + dihydrobiopterin + H2O
-
?
1.14.16.1 L-phenylalanine + tetrahydrobiopterin + O2 relatively low activity with tetrahydrobiopterin can be selectively increased by limited proteolysis Rattus norvegicus L-tyrosine + dihydrobiopterin + H2O
-
?
1.14.16.1 L-phenylalanine + tetrahydrobiopterin + O2 low activity with tetrahydrobiopterin can be selectively increased by a wide variety of reversible and irreversible modificators of the enzyme, e.g. interaction with phospholipids Homo sapiens L-tyrosine + dihydrobiopterin + H2O
-
?
1.14.16.1 L-phenylalanine + tetrahydrobiopterin + O2 low activity with tetrahydrobiopterin can be selectively increased by a wide variety of reversible and irreversible modificators of the enzyme, e.g. interaction with phospholipids Rattus norvegicus L-tyrosine + dihydrobiopterin + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.14.16.1 dimer
-
Rattus norvegicus
1.14.16.1 dimer 2 * 54000, fetal liver enzyme, SDS-PAGE Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.16.1 tetrahydrobiopterin
-
Homo sapiens
1.14.16.1 tetrahydrobiopterin
-
Rattus norvegicus