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Literature summary extracted from

  • Villanova, E.; Manjon, A.; Iborra, J.L.
    Tyrosine hydroxylase activity of immobilized tyrosinase on Enzyacryl-AA and CPG-AA supports: stabilization and properties (1984), Biotechnol. Bioeng., 26, 1306-1312.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.16.2 synthesis immobilization of tyrosinase on polyacrylamide-based support for production of L-dopa from L-tyrosine thereby modifying the enzyme activity to tyrosine hydroxylase Pelophylax lessonae

General Stability

EC Number General Stability Organism
1.14.16.2 enzyme is stabilized by immobilization on polyacrylamide-based support Pelophylax lessonae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.16.2 L-tyrosine + tetrahydrobiopterin + O2 Pelophylax lessonae first step in biosynthesis of catecholamines such as norepinephrine, epinephrine and dopamine 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
 ?
1.14.16.2 L-tyrosine + tetrahydrobiopterin + O2 Pelophylax lessonae reaction is coupled with ascorbate oxidation 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.2 Pelophylax lessonae
-
 ridibunda, frog
-
1.14.16.2 Pelophylax lessonae
-
 tyrosinase activity is modified to tyrosine hydroxylase activity via immobilization
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.16.2 immobilization on polyacrylamide-based support Pelophylax lessonae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.16.2 epidermis
-
 Pelophylax lessonae
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.16.2 additional information
-
 activity of immobilized enzyme under different conditions in bioreactors Pelophylax lessonae
1.14.16.2 3.46
-
 purified enzyme, immobilized Pelophylax lessonae

Storage Stability

EC Number Storage Stability Organism
1.14.16.2 -30°C, immobilized and lyophilized enzyme, stored in dry environment, unaltered more than 150 days Pelophylax lessonae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.2 L-tyrosine + ascorbate + O2
-
 Pelophylax lessonae 3,4-dihydroxy-L-phenylalanine + dehydroascorbate + H2O
-
 ?
1.14.16.2 L-tyrosine + tetrahydrobiopterin + O2
-
 Pelophylax lessonae 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O 3,4-dihydroxy-L-phenylalanine is identical with dopa ?
1.14.16.2 L-tyrosine + tetrahydrobiopterin + O2 first step in biosynthesis of catecholamines such as norepinephrine, epinephrine and dopamine Pelophylax lessonae 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
 ?
1.14.16.2 L-tyrosine + tetrahydrobiopterin + O2 reaction is coupled with ascorbate oxidation Pelophylax lessonae 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
 ?
1.14.16.2 additional information tyrosinase activity is modified to tyrosine hydroxylase activity via immobilization Pelophylax lessonae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.16.2 monophenol monooxygenase
-
 Pelophylax lessonae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.16.2 25
-
 assay at Pelophylax lessonae