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Literature summary extracted from

  • Jaron, S.; Mains, R.E.; Eipper, B.A.; Blackburn, N.J.
    The catalytic role of the copper ligand H172 of peptidylglycine alpha-hydroxylating monooxygenase (PHM): a spectroscopic study of the H172A mutant (2002), Biochemistry, 41, 13274-13282.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.17.3 medullary thyroid alpha-AE, type A, expression in chinese hamster ovary cells Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
1.14.17.3 H172A mutant of copper ligand of peptidylglycine alpha-hydroxylating enzyme, reduced copper content below 0.3 Cu2+ per protein molecule Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.17.3 Cu2+ mutant H172A has reduced copper content: below 0.3 Cu2+ per protein molecule Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.14.17.3 Rattus norvegicus
-
bifunctional alpha-AE
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.17.3 mutant H172A of type A alpha-AE, recombinant from chinese hamster ovary cells Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.17.3 [peptide]-glycine + 2 ascorbate + O2 = [peptide]-(2S)-2-hydroxyglycine + 2 monodehydroascorbate + H2O structural analysis of mutant H172A, binding of ligands CO, Cu2+ Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.17.3 medullary thyroid carcinoma cell
-
Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.17.3 monoiodo-alpha-N-acetyl-Tyr-Val-Gly + ascorbate + O2
-
Rattus norvegicus monoiodo-alpha-N-acetyl-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O
-
?
1.14.17.3 peptidylglycine + ascorbate + O2 requirement for O2 Rattus norvegicus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
?
1.14.17.3 peptidylglycine + ascorbate + O2 COOH-terminal glycine Rattus norvegicus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.17.3 ascorbate
-
Rattus norvegicus