EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.17.3 | cytoplasm | - |
Rattus norvegicus | 5737 | - |
1.14.17.3 | membrane | integral membrane | Rattus norvegicus | 16020 | - |
1.14.17.3 | membrane | from neurosecretory vesicles | Rattus norvegicus | 16020 | - |
1.14.17.3 | additional information | distribution of membrane-bound and soluble peptidylglycine alpha-hydroxylating activity | Rattus norvegicus | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.17.3 | Cu2+ | - |
Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.17.3 | additional information | - |
- |
Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.17.3 | peptidylglycine + ascorbate + O2 | Rattus norvegicus | - |
peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.17.3 | Rattus norvegicus | - |
PAM-1 and PAM-2 | - |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.14.17.3 | [peptide]-glycine + 2 ascorbate + O2 = [peptide]-(2S)-2-hydroxyglycine + 2 monodehydroascorbate + H2O | sequential activity of 2 enzymes contained within a bifunctional protein | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.17.3 | hippocampus | PAM-1 and PAM-2 | Rattus norvegicus | - |
1.14.17.3 | hypothalamus | PAM-1 and PAM-2 | Rattus norvegicus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.17.3 | alpha-N-acetyl-Tyr-Val-Gly + ascorbate + O2 | - |
Rattus norvegicus | alpha-N-acetyl-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O | - |
? | |
1.14.17.3 | monoiodo-alpha-N-acetyl-Tyr-Val-Gly + ascorbate + O2 | - |
Rattus norvegicus | monoiodo-alpha-N-acetyl-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O | - |
? | |
1.14.17.3 | additional information | EC 1.14.17.3 is often called peptidylglycine alpha-amidating monooxygenase (PAM) and the alpha-amidated product is mentioned as the product of the reaction, but the alpha-amidation of glycine-extended peptides is a two-step process catalyzed by 2 enzymes: 1. EC 1.14.17.3: production of peptidyl(2-hydroxyglycine) by a copper, molecular oxygen and ascorbate-dependent peptidyl-glycine alpha-hydroxylating monooxygenase (PMH) and 2. conversion of the peptidyl-alpha-hydroxyglycine derivative into an alpha-amidated product at physiological pH by peptidyl-alpha-hydroxyglycine alpha-amidating lyase | Rattus norvegicus | ? | - |
? | |
1.14.17.3 | peptidylglycine + ascorbate + O2 | - |
Rattus norvegicus | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
1.14.17.3 | peptidylglycine + ascorbate + O2 | COOH-terminal glycine | Rattus norvegicus | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.17.3 | More | EC 1.14.17.3 is often called peptidylglycine alpha-amidating monooxygenase (PAM) and the alpha-amidated product is mentioned as the product of the reaction, but the alpha-amidation of glycine-extended peptides is a two-step process catalyzed by 2 enzymes: 1. EC 1.14.17.3: production of peptidyl(2-hydroxyglycine) by a copper, molecular oxygen and ascorbate-dependent peptidyl-glycine alpha-hydroxylating monooxygenase (PHM), 2. conversion of the peptidyl-alpha-hydroxyglycine derivative into an alpha-amidated product at physiological pH by peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PHL) | Rattus norvegicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.17.3 | 37 | - |
assay at | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.17.3 | 4 | 4.5 | membrane-bound peptidylglycine-alpha-hydroxylating activity | Rattus norvegicus |
1.14.17.3 | 4.5 | 5 | soluble peptidylglycine-alpha-hydroxylating activity | Rattus norvegicus |
1.14.17.3 | 5 | - |
assay at | Rattus norvegicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.17.3 | ascorbate | dependent on | Rattus norvegicus |