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Literature summary extracted from
- Peptide C-terminal alpha-amidating enzyme purified to homogeneity from Xenopus laevis skin (1986), Biochem. Biophys. Res. Commun., 137, 984-991.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.17.3 | dithiothreitol | - |
Xenopus laevis |  |
| 1.14.17.3 | EDTA | - |
Xenopus laevis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.17.3 | 0.00035 | - |
acetyl-L-Tyr-L-Phe-Gly | in presence of 0.25 mM ascorbate | Xenopus laevis |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.17.3 | Cu2+ | a copper protein | Xenopus laevis | |
| 1.14.17.3 | Cu2+ | Cu2+ is absolutely required for optimal activity | Xenopus laevis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.17.3 | 39000 | - |
SDS-PAGE, gel filtration | Xenopus laevis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.17.3 | peptidylglycine + ascorbate + O2 | Xenopus laevis | - |
peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.17.3 | Xenopus laevis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.17.3 | - |
Xenopus laevis |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.17.3 | skin | - |
Xenopus laevis | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.17.3 | additional information | - |
- |
Xenopus laevis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.17.3 | acetyl-L-Tyr-L-Phe-Gly + ascorbate + O2 | - |
Xenopus laevis | acetyl-L-Tyr-L-Phe-2-hydroxyglycine + dehydroascorbate + H2O | - |
r | |
| 1.14.17.3 | peptidylglycine + ascorbate + O2 | - |
Xenopus laevis | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
| 1.14.17.3 | peptidylglycine + ascorbate + O2 | diverse synthetic substrates with C-terminal glycine | Xenopus laevis | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
| 1.14.17.3 | peptidylglycine + ascorbate + O2 | COOH-terminal glycine | Xenopus laevis | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.17.3 | monomer | 1 * 39000, SDS-PAGE | Xenopus laevis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.17.3 | 37 | - |
assay at | Xenopus laevis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.17.3 | 6 | 7 | - |
Xenopus laevis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.17.3 | ascorbate | dependent on | Xenopus laevis | |
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