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Literature summary extracted from

  • Glembotski, C.C.; Eipper, B.A.; Mains, R.E.
    Characterization of a peptide alpha-amidation activity from rat anterior pituitary (1984), J. Biol. Chem., 259, 6385-6392.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.17.3 adrenocorticotrophic hormone(1-10)
-
 Rattus norvegicus
1.14.17.3 alpha-N-acetyl-adrenocorticotrophic hormone(1-14)
-
 Rattus norvegicus
1.14.17.3 endorphin(51-61)NH2 i.e. pro-adrenocorticotrophic hormone(1-10) Rattus norvegicus
1.14.17.3 endorphins and proendorphins Rattus norvegicus
1.14.17.3 peptides especially those with COOH-terminal glycine residues Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.17.3 secretory granule
-
 Rattus norvegicus 30141
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.17.3 Cu2+ Cu2+ strongly stimulates activity Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.17.3 peptidylglycine + ascorbate + O2 Rattus norvegicus
-
 peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.17.3 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.17.3 pituitary gland anterior Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.17.3 additional information
-
 very low activity in crude subcellular preparations Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.17.3 alpha-N-acetyl-adrenocorticotrophic hormone(1-14) + ascorbate + O2 inhibitory substrate Rattus norvegicus alpha-N-acetyl-adrenocorticotrophic hormone(1-13)-2-hydroxyglycine + dehydroascorbate + H2O
-
 r
1.14.17.3 D-Tyr-Val-Gly + ascorbate + O2 synthetic peptid Rattus norvegicus D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O
-
 ?
1.14.17.3 monoiodo-D-Tyr-Val-Gly + ascorbate + O2 synthetic peptid Rattus norvegicus monoiodo-D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O
-
 ?
1.14.17.3 additional information specificity Rattus norvegicus ?
-
 ?
1.14.17.3 additional information endorphins are inhibitory substrates Rattus norvegicus ?
-
 ?
1.14.17.3 peptidylglycine + ascorbate + O2
-
 Rattus norvegicus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?
1.14.17.3 peptidylglycine + ascorbate + O2 requirement for O2 Rattus norvegicus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?
1.14.17.3 peptidylglycine + ascorbate + O2 COOH-terminal glycine Rattus norvegicus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.17.3 6.5 7
-
 Rattus norvegicus
1.14.17.3 7
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.17.3 6 7.5 about 70% of activity maximum at pH 6.0 and 7.5 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.17.3 ascorbate dependent on Rattus norvegicus
1.14.17.3 FAD slight activity with Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.17.3 0.0006
-
 endorphin(51-61)NH2
-
 Rattus norvegicus
1.14.17.3 0.001
-
 alpha-N-acetyl-adrenocorticotrophic hormone(1-14)
-
 Rattus norvegicus
1.14.17.3 0.005
-
 adrenocorticotrophic hormone (1-10)
-
 Rattus norvegicus