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Literature summary extracted from
- Recombinant type A rat 75-kDa alpha-amidating enzyme catalyzes the conversion of glycine-extended peptides to peptide amides via an alpha-hydroxyglycine intermediate (1991), Arch. Biochem. Biophys., 289, 192-196.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.17.3 | truncated type A enzyme, expression in mouse C127 cells | Rattus norvegicus |
| 4.3.2.5 | - |
Rattus norvegicus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.17.3 | additional information | - |
additional information | different assay methods | Rattus norvegicus | |
| 4.3.2.5 | 0.0023 | 0.004 | dansyl-Tyr-Val-Gly | 37°C, pH 6 | Rattus norvegicus |  |
| 4.3.2.5 | 0.035 | 0.048 | Dansyl-Tyr-Val-alpha-hydroxyglycine | 37°C, pH 7.0 | Rattus norvegicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.3.2.5 | extracellular | - |
Rattus norvegicus | - |
- |
| 4.3.2.5 | soluble | - |
Rattus norvegicus | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.17.3 | Cu2+ | Cu2+ is absolutely required for optimal activity | Rattus norvegicus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.17.3 | 43000 | - |
recombinant form B, gel filtration and SDS-PAGE | Rattus norvegicus |
| 1.14.17.3 | 75000 | - |
recombinant form A, gel filtration and SDS-PAGE | Rattus norvegicus |
| 4.3.2.5 | 75000 | - |
truncated type A enzyme which catalyzes a two-step reaction involving an initial hydroxylation of peptidyl-Gly followed by conversion of the peptidyl-alpha-hydroxyglycine intermediate to the amidated product | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.17.3 | peptidylglycine + ascorbate + O2 | Rattus norvegicus | - |
peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.17.3 | Rattus norvegicus | - |
2 forms: type A and B | - |
| 4.3.2.5 | Rattus norvegicus | - |
truncated type A enzyme which catalyzes a two-step reaction involving an initial hydroxylation of peptidyl-Gly followed by conversion of the peptidyl-alpha-hydroxyglycine intermediate to the amidated product, enzyme is secreted into the medium from mouse C127 cells transfected with the rat MTC cDNA encoding the truncated type A enzyme | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.17.3 | recombinant from mouse cells | Rattus norvegicus |
| 1.14.17.3 | type A and B | Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.3.2.5 | medullary thyroid carcinoma cell | enzyme is secreted into the medium from mouse C127 cells transfected with the rat MTC cDNA encoding the truncated type A enzyme | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.3.2.5 | 0.25 | 0.32 | dansyl-Tyr-Val-Gly as substrate | Rattus norvegicus |
| 4.3.2.5 | 4.5 | 8.3 | dansyl-Tyr-Val-alpha-hydroxyglycine as substrate | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.17.3 | dansyl-D-Tyr-Val-Gly + ascorbate + O2 | stereospecific | Rattus norvegicus | dansyl-D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O | - |
? | |
| 1.14.17.3 | peptidylglycine + ascorbate + O2 | - |
Rattus norvegicus | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
| 1.14.17.3 | peptidylglycine + ascorbate + O2 | COOH-terminal glycine | Rattus norvegicus | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
| 4.3.2.5 | dansyl-Tyr-Val-alpha-hydroxyglycine | - |
Rattus norvegicus | dansyl-Tyr-Val-NH2 + glyoxylate | - |
? | |
| 4.3.2.5 | dansyl-Tyr-Val-Gly | - |
Rattus norvegicus | dansyl-Tyr-Val-NH2 + glyoxylate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.17.3 | monomer | 1 * 75000, type A, SDS-PAGE | Rattus norvegicus |
| 1.14.17.3 | monomer | 1 * 43000, type B, SDS-PAGE | Rattus norvegicus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.17.3 | More | the 2-step process is catalyzed by only 1 enzyme called type A peptidylglycine alpha-amidating enzyme | Rattus norvegicus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.3.2.5 | 37 | - |
assay at | Rattus norvegicus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.17.3 | ascorbate | dependent on | Rattus norvegicus | |
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