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Literature summary extracted from

  • Beaudry, G.A.; Mehta, N.M.; Ray, M.I.; Bertelsen, A.H.
    Purification and characterization of functional recombinant alpha-amidating enzyme secreted from mammalian cells (1990), J. Biol. Chem., 265, 17694-17699.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.17.3 medullary thyroid carcinoma enzyme, expression in mouse C 127 cells via bovine papilloma virus vector, amino acid and DNA sequence analysis Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.17.3 0.003
-
 dansyl-Tyr-Val-Gly recombinant enzyme Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.17.3 75000
-
 recombinant from mouse cells, gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.17.3 peptidylglycine + ascorbate + O2 Rattus norvegicus
-
 peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.17.3 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.14.17.3 glycoprotein partially glycosylated, at Asn 660, recombinant enzyme from mouse cells Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.17.3 recombinant from mouse cells Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.17.3 pituitary gland
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.17.3 1.15
-
 recombinant purified enzyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.17.3 dansyl-D-Tyr-Val-Gly + ascorbate + O2
-
 Rattus norvegicus dansyl-D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O
-
 ?
1.14.17.3 peptidylglycine + ascorbate + O2
-
 Rattus norvegicus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?
1.14.17.3 peptidylglycine + ascorbate + O2 COOH-terminal glycine Rattus norvegicus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.17.3 monomer 1 * 75000, recombinant enzyme, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.17.3 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.17.3 4.5 5 recombinant enzyme Rattus norvegicus
1.14.17.3 7
-
 assay at, recombinant enzyme Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.17.3 4 9 recombinant enzyme Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.17.3 additional information
-
 rapid inactivation of recombinant enzyme at acidic pH in vitro Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.17.3 ascorbate dependent on Rattus norvegicus