Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bradbury, A.F.; Smyth, D.G.
    Enzyme-catalysed peptide amidation. Isolation of a stable intermediate formed by reaction of the amidating enzyme with an imino acid (1987), Eur. J. Biochem., 169, 579-584.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.17.3 D-Tyr-Val-Gly at 2 mM 82% inhibition, at 0.1 mM 45% inhibition Sus scrofa
1.14.17.3 Glyoxylate 2-pyridylhydrazone
-
Sus scrofa
1.14.17.3 Glyoxylate 4-carboxyphenylhydrazone
-
Sus scrofa
1.14.17.3 Glyoxylate benzylhydrazone
-
Sus scrofa
1.14.17.3 glyoxylate phenylhydrazone strongly Sus scrofa
1.14.17.3 Glyoxylate phenylsemicarbazone
-
Sus scrofa
1.14.17.3 Glyoxylate phenylthiosemicarbazone
-
Sus scrofa
1.14.17.3 Glyoxylate semicarbazone
-
Sus scrofa
1.14.17.3 glyoxylate thiosemicarbazone
-
Sus scrofa
1.14.17.3 glyoxylate-N-hexyl-4-carbamoylphenylhydrazone strongly Sus scrofa
1.14.17.3 N-Carboxymethyl N'-phenylhydrazone weak inhibition Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.17.3 40000
-
gel fltration, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.17.3 peptidylglycine + ascorbate + O2 Sus scrofa
-
peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.17.3 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.17.3
-
Sus scrofa

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.17.3 [peptide]-glycine + 2 ascorbate + O2 = [peptide]-(2S)-2-hydroxyglycine + 2 monodehydroascorbate + H2O mechanism Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.17.3 pituitary gland
-
Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.17.3 D-Tyr-Val-Gly + ascorbate + O2
-
Sus scrofa D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O
-
?
1.14.17.3 glyoxylic acid phenylhydrazone + ascorbate + O2 competitive substrate, inhibitory Sus scrofa oxalate phenylhydrazide + dehydroascorbate + H2O
-
?
1.14.17.3 additional information substrates are also physiologically relevant peptides related to alpha-melanotropine Sus scrofa ?
-
?
1.14.17.3 peptidylglycine + ascorbate + O2
-
Sus scrofa peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
?
1.14.17.3 peptidylglycine + ascorbate + O2 COOH-terminal glycine Sus scrofa peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.14.17.3 monomer 1 * 40000, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.17.3 37
-
assay at Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.17.3 ascorbate dependent on Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.17.3 0.015
-
glyoxylate phenylhydrazone
-
Sus scrofa
1.14.17.3 0.11
-
glyoxylate thiosemicarbazone
-
Sus scrofa