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Literature summary extracted from

  • Twilfer,H.; Sandfort, G; Bernhardt, F.H.
    Substrate and solvent isotope effects on the fate of the active oxygen species in substrate-modulated reactions of putidamonooxin (2000), Eur. J. Biochem., 267, 5926-5934.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.99.15 0.0062
-
 4-Methylbenzoate reaction in D2O Pseudomonas putida
1.14.99.15 0.009
-
 4-Methylbenzoate reaction in H2O Pseudomonas putida
1.14.99.15 0.0144
-
 4-trifluoromethylbenzoate reaction in D2O Pseudomonas putida
1.14.99.15 0.024
-
 4-trifluoromethylbenzoate reaction in H2O Pseudomonas putida
1.14.99.15 0.0367
-
 benzoate reaction in H2O Pseudomonas putida
1.14.99.15 0.055
-
 benzoate reaction in D2O Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.99.15 4-methoxybenzoate + NADH + O2 Pseudomonas putida
-
 4-hydroxybenzoate + NAD+ + H2O + formaldehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.15 Pseudomonas putida
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.99.15 194.5
-
 3-phenyl-4-[2H3]-methoxybenzoate, product formation, reaction in H2O Pseudomonas putida
1.14.99.15 207.8
-
 4-trifluoromethylbenzoate, oxygen uptake, reaction in D2O Pseudomonas putida
1.14.99.15 239.8
-
 3-phenyl-4-[2H3]-methoxybenzoate, product formation, reaction in D2O Pseudomonas putida
1.14.99.15 265.6
-
 benzoate, oxygen uptake, reaction in D2O Pseudomonas putida
1.14.99.15 295.1
-
 benzoate, oxygen uptake, reaction in H2O Pseudomonas putida
1.14.99.15 303.9
-
 3-phenyl-4-[1H3]-methoxybenzoate, product formation, reaction in H2O Pseudomonas putida
1.14.99.15 305.2
-
 4-methylbenzoate, NADH oxidation, reaction in H2O Pseudomonas putida
1.14.99.15 310.1
-
 3-phenyl-4-[2H3]-methoxybenzoate, oxygen uptake, reaction in D2O Pseudomonas putida
1.14.99.15 310.1
-
 3-phenyl-4-[2H3]-methoxybenzoate, oxygen uptake, reaction in H2O Pseudomonas putida
1.14.99.15 315.5
-
 4-trifluoromethylbenzoate, NADH oxidation, reaction in D2O Pseudomonas putida
1.14.99.15 317.2
-
 4-methylbenzoate, NADH oxidation, reaction in D2O Pseudomonas putida
1.14.99.15 317.4
-
 3-phenyl-4-[1H3]-methoxybenzoate, product formation, reaction in D2O Pseudomonas putida
1.14.99.15 326.7
-
 3-phenyl-4-[1H3]-methoxybenzoate, oxygen uptake, reaction in H2O Pseudomonas putida
1.14.99.15 329.2
-
 3-phenyl-4-[1H3]-methoxybenzoate, oxygen uptake, reaction in D2O Pseudomonas putida
1.14.99.15 339.1
-
 benzoate, NADH oxidation, reaction in D2O Pseudomonas putida
1.14.99.15 340.2
-
 3-phenyl-4-[2H3]-methoxybenzoate, NADH oxidation, reaction in H2O Pseudomonas putida
1.14.99.15 345
-
 4-trifluoromethylbenzoate, oxygen uptake, reaction in H2O Pseudomonas putida
1.14.99.15 345.6
-
 benzoate, NADH oxidation, reaction in H2O Pseudomonas putida
1.14.99.15 353.5
-
 3-phenyl-4-[2H3]-methoxybenzoate, NADH oxidation, reaction in D2O Pseudomonas putida
1.14.99.15 360.4
-
 3-phenyl-4-[1H3]-methoxybenzoate, NADH oxidation, reaction in H2O Pseudomonas putida
1.14.99.15 367
-
 4-methylbenzoate, oxygen uptake, reaction in H2O Pseudomonas putida
1.14.99.15 368
-
 4-methylbenzoate, oxygen uptake, reaction in D2O Pseudomonas putida
1.14.99.15 380.2
-
 3-phenyl-4-[1H3]-methoxybenzoate, NADH oxidation, reaction in D2O Pseudomonas putida
1.14.99.15 391.5
-
 4-methoxybenzoate, oxygen uptake, reaction in D2O Pseudomonas putida
1.14.99.15 394.1
-
 4-methoxybenzoate, oxygen uptake, reaction in H2O Pseudomonas putida
1.14.99.15 402.6
-
 3-nitro-4-methoxybenzoate, NADH oxidation, reaction in D2O Pseudomonas putida
1.14.99.15 409.7
-
 4-trifluoromethylbenzoate, NADH oxidation, reaction in H2O Pseudomonas putida
1.14.99.15 422.1
-
 3-nitro-4-methoxybenzoate, NADH oxidation, reaction in H2O Pseudomonas putida
1.14.99.15 432.4
-
 3-nitro-4-methoxybenzoate, oxygen uptake, reaction in H2O Pseudomonas putida
1.14.99.15 432.7
-
 3-nitro-4-methoxybenzoate, oxygen uptake, reaction in D2O Pseudomonas putida
1.14.99.15 442.2
-
 4-methoxybenzoate, NADH oxidation, reaction in D2O Pseudomonas putida
1.14.99.15 445
-
 4-methoxybenzoate, NADH oxidation, reaction in H2O Pseudomonas putida
1.14.99.15 450.9
-
 3-nitro-4-methoxybenzoate, product formation, reaction in H2O Pseudomonas putida
1.14.99.15 487.3
-
 3-nitro-4-methoxybenzoate, product formation, reaction in D2O Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.15 3-nitro-4-methoxybenzoate + NADH + O2
-
 Pseudomonas putida 3-nitro-4-hydroxybenzoate + NAD+ + H2O + formaldehyde
-
 ?
1.14.99.15 3-phenyl-4-methoxybenzoate + NADH + O2
-
 Pseudomonas putida 3-phenyl-4-hydroxybenzoate + NAD+ + H2O + formaldehyde
-
 ?
1.14.99.15 4-methoxybenzoate + NADH + O2
-
 Pseudomonas putida 4-hydroxybenzoate + NAD+ + H2O + formaldehyde
-
 ?
1.14.99.15 4-methylbenzoate + NADH + O2
-
 Pseudomonas putida 4-carboxybenzylalcohol + NAD+ + H2O
-
 ?
1.14.99.15 4-trifluoromethylbenzoate + NADH + O2
-
 Pseudomonas putida ?
-
 ?
1.14.99.15 benzoate + NADH + O2
-
 Pseudomonas putida ?
-
 ?