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Literature summary extracted from

  • Kang, H.K.; Lee, S.S.
    Heterogeneous natures of the microbial steroid 9alpha-hydroxylase in nocardioforms (1997), Arch. Pharm. Res., 20, 519-524.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.99.24 synthesis enzyme occurs in many bacterial genera used in industrial processes Corynebacterium sp.
1.14.99.24 synthesis enzyme occurs in many bacterial genera used in industrial processes Mycobacterium sp.
1.14.99.24 synthesis enzyme occurs in many bacterial genera used in industrial processes Nocardia sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.99.24 2,2'-bipyridine
-
 Corynebacterium sp.
1.14.99.24 2,2'-bipyridine
-
 Nocardia sp.
1.14.99.24 2,2'-bipyridine
-
 Rhodococcus erythropolis
1.14.99.24 8-hydroxyquinoline
-
 Corynebacterium sp.
1.14.99.24 8-hydroxyquinoline
-
 Mycobacterium sp.
1.14.99.24 8-hydroxyquinoline
-
 Rhodococcus erythropolis
1.14.99.24 8-hydroxyquinoline
-
 Rhodococcus rhodochrous
1.14.99.24 Metyrapone
-
 Mycobacterium sp.
1.14.99.24 o-phenanthroline
-
 Corynebacterium sp.
1.14.99.24 o-phenanthroline
-
 Mycobacterium sp.
1.14.99.24 o-phenanthroline
-
 Rhodococcus erythropolis
1.14.99.24 o-phenanthroline
-
 Rhodococcus rhodochrous
1.14.99.24 Sodium cyanide
-
 Corynebacterium sp.
1.14.99.24 Sodium cyanide
-
 Mycobacterium sp.
1.14.99.24 Sodium cyanide
-
 Rhodococcus erythropolis
1.14.99.24 Sodium cyanide
-
 Rhodococcus rhodochrous

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.99.24 cytoplasm
-
 Corynebacterium sp. 5737
-
1.14.99.24 cytoplasm
-
 Nocardia sp. 5737
-
1.14.99.24 cytoplasm
-
 Rhodococcus sp. 5737
-
1.14.99.24 cytosol
-
 Mycobacterium sp. 5829
-
1.14.99.24 cytosol
-
 Nocardia sp. 5829
-
1.14.99.24 cytosol
-
 Mycolicibacterium fortuitum 5829
-
1.14.99.24 membrane
-
 Corynebacterium sp. 16020
-
1.14.99.24 membrane
-
 Nocardia sp. 16020
-
1.14.99.24 membrane
-
 Rhodococcus sp. 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.99.24 4,9(11)-pregnadiene-3,20-dione + NADH + O2 Rhodococcus rhodochrous
-
 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?
1.14.99.24 4,9(11)-pregnadiene-3,20-dione + NADH + O2 Corynebacterium sp.
-
 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?
1.14.99.24 4,9(11)-pregnadiene-3,20-dione + NADH + O2 Mycobacterium sp.
-
 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?
1.14.99.24 4,9(11)-pregnadiene-3,20-dione + NADH + O2 Nocardia sp.
-
 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?
1.14.99.24 4,9(11)-pregnadiene-3,20-dione + NADH + O2 Rhodococcus sp.
-
 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?
1.14.99.24 4,9(11)-pregnadiene-3,20-dione + NADH + O2 Rhodococcus erythropolis
-
 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?
1.14.99.24 4,9(11)-pregnadiene-3,20-dione + NADH + O2 Mycolicibacterium fortuitum
-
 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?
1.14.99.24 4,9(11)-pregnadiene-3,20-dione + NADH + O2 Nocardia sp. M117
-
 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?
1.14.99.24 4,9(11)-pregnadiene-3,20-dione + NADH + O2 Rhodococcus rhodochrous KCTC 1061 / ATCC 17895
-
 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?
1.14.99.24 4,9(11)-pregnadiene-3,20-dione + NADH + O2 Rhodococcus erythropolis KCTC 1062
-
 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.24 Corynebacterium sp.
-
 synonym Nocardia restrictus, ATCC 14887 (MCI 1354)
-
1.14.99.24 Mycobacterium sp.
-
-
-
1.14.99.24 Mycolicibacterium fortuitum
-
 KCTC 1122, ATCC 6842
-
1.14.99.24 Nocardia sp.
-
-
-
1.14.99.24 Nocardia sp. M117
-
-
-
1.14.99.24 Rhodococcus erythropolis
-
 synonym Nocardia erythropolis
-
1.14.99.24 Rhodococcus erythropolis KCTC 1062
-
 synonym Nocardia erythropolis
-
1.14.99.24 Rhodococcus rhodochrous
-
 synonym Nocardia erythropolis
-
1.14.99.24 Rhodococcus rhodochrous KCTC 1061 / ATCC 17895
-
 synonym Nocardia erythropolis
-
1.14.99.24 Rhodococcus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.99.24
-
 Nocardia sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.24 4,9(11)-pregnadiene-3,20-dione + NADH + O2
-
 Rhodococcus rhodochrous 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?
1.14.99.24 4,9(11)-pregnadiene-3,20-dione + NADH + O2
-
 Corynebacterium sp. 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?
1.14.99.24 4,9(11)-pregnadiene-3,20-dione + NADH + O2
-
 Mycobacterium sp. 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?
1.14.99.24 4,9(11)-pregnadiene-3,20-dione + NADH + O2
-
 Nocardia sp. 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?
1.14.99.24 4,9(11)-pregnadiene-3,20-dione + NADH + O2
-
 Rhodococcus sp. 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?
1.14.99.24 4,9(11)-pregnadiene-3,20-dione + NADH + O2
-
 Rhodococcus erythropolis 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?
1.14.99.24 4,9(11)-pregnadiene-3,20-dione + NADH + O2
-
 Mycolicibacterium fortuitum 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?
1.14.99.24 4,9(11)-pregnadiene-3,20-dione + NADH + O2
-
 Nocardia sp. M117 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?
1.14.99.24 4,9(11)-pregnadiene-3,20-dione + NADH + O2
-
 Rhodococcus rhodochrous KCTC 1061 / ATCC 17895 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?
1.14.99.24 4,9(11)-pregnadiene-3,20-dione + NADH + O2
-
 Rhodococcus erythropolis KCTC 1062 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?
1.14.99.24 9(11)-dehydro-17alpha-methyl-testosterone + NADH + O2
-
 Rhodococcus rhodochrous 9alpha,11alpha-oxido-17beta-hydroxy-17alpha-methyl-4-androstene-3-one + 9alpha,11alpha-oxido-17beta-hydroxy-17alpha-methyl-1,4-androstadiene-3-one + NAD+ + H2O
-
 ?
1.14.99.24 9(11)-dehydro-17alpha-methyl-testosterone + NADH + O2
-
 Corynebacterium sp. 9alpha,11alpha-oxido-17beta-hydroxy-17alpha-methyl-4-androstene-3-one + 9alpha,11alpha-oxido-17beta-hydroxy-17alpha-methyl-1,4-androstadiene-3-one + NAD+ + H2O
-
 ?
1.14.99.24 9(11)-dehydro-17alpha-methyl-testosterone + NADH + O2
-
 Mycobacterium sp. 9alpha,11alpha-oxido-17beta-hydroxy-17alpha-methyl-4-androstene-3-one + 9alpha,11alpha-oxido-17beta-hydroxy-17alpha-methyl-1,4-androstadiene-3-one + NAD+ + H2O
-
 ?
1.14.99.24 9(11)-dehydro-17alpha-methyl-testosterone + NADH + O2
-
 Rhodococcus sp. 9alpha,11alpha-oxido-17beta-hydroxy-17alpha-methyl-4-androstene-3-one + 9alpha,11alpha-oxido-17beta-hydroxy-17alpha-methyl-1,4-androstadiene-3-one + NAD+ + H2O
-
 ?
1.14.99.24 9(11)-dehydro-17alpha-methyl-testosterone + NADH + O2
-
 Rhodococcus erythropolis 9alpha,11alpha-oxido-17beta-hydroxy-17alpha-methyl-4-androstene-3-one + 9alpha,11alpha-oxido-17beta-hydroxy-17alpha-methyl-1,4-androstadiene-3-one + NAD+ + H2O
-
 ?
1.14.99.24 9(11)-dehydro-17alpha-methyl-testosterone + NADH + O2
-
 Rhodococcus rhodochrous KCTC 1061 / ATCC 17895 9alpha,11alpha-oxido-17beta-hydroxy-17alpha-methyl-4-androstene-3-one + 9alpha,11alpha-oxido-17beta-hydroxy-17alpha-methyl-1,4-androstadiene-3-one + NAD+ + H2O
-
 ?
1.14.99.24 9(11)-dehydro-17alpha-methyl-testosterone + NADH + O2
-
 Rhodococcus erythropolis KCTC 1062 9alpha,11alpha-oxido-17beta-hydroxy-17alpha-methyl-4-androstene-3-one + 9alpha,11alpha-oxido-17beta-hydroxy-17alpha-methyl-1,4-androstadiene-3-one + NAD+ + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.99.24 cytochrome P450
-
 Mycobacterium sp.
1.14.99.24 NADH
-
 Mycobacterium sp.
1.14.99.24 NADH
-
 Nocardia sp.
1.14.99.24 NADH
-
 Mycolicibacterium fortuitum
1.14.99.24 NADPH
-
 Rhodococcus rhodochrous
1.14.99.24 NADPH
-
 Nocardia sp.
1.14.99.24 NADPH
-
 Rhodococcus sp.
1.14.99.24 NADPH
-
 Rhodococcus erythropolis