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Literature summary extracted from

  • Abbruzzese, A.; Hanauske-Abel, H.M.; Park, M.H.; Henke, S.; Folk, J.E.
    The active site of deoxyhypusyl hydroxylase: use of catecholpeptides and their component chelator and peptide moieties as molecular probes (1991), Biochim. Biophys. Acta, 1077, 159-166.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.99.29 2,3-Dihydroxybenzoic acid slight inhibition at 2 mM Rattus norvegicus
1.14.99.29 3,4-dihydroxybenzoic acid above 50% inhibition at 2 mM Rattus norvegicus
1.14.99.29 ethyl 3,4-dihydroxybenzoate IC50 0.5 mM Rattus norvegicus
1.14.99.29 methyl 2,3-dihydroxybenzoate IC50 1.6 mM Rattus norvegicus
1.14.99.29 N1-acetyl-L-Orn-L-Pro-Gly above 2 mM Rattus norvegicus
1.14.99.29 N1-acetyl-N4-(2,3-dihydroxybenzoyl)-L-Orn-L-Pro-Gly IC50 0.2 mM Rattus norvegicus
1.14.99.29 N1-acetyl-N4-(3,4-dihydroxybenzoyl)-L-Orn-L-Pro-Gly IC50 0.03 mM Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.29 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.99.29 testis
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.99.29 additional information
-
 corresponds to 2.8 pmol/min/mg for partially purified enzyme Rattus norvegicus
1.14.99.29 416
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.29 N'-(4-aminobutyl)lysine + electron donor + O2 i.e. deoxyhypusine Rattus norvegicus N'-(4-amino-2-hydroxybutyl)lysine + oxidized electron donor + H2O i.e. hypusine ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.99.29 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.99.29 7 7.5 in 20 mM Tris buffer Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.99.29 0.032
-
 N1-acetyl-N4-(3,4-dihydroxybenzoyl)-L-Orn-L-Pro-Gly competitive inhibition Rattus norvegicus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.14.99.29 0.03
-
 IC50 0.03 mM Rattus norvegicus N1-acetyl-N4-(3,4-dihydroxybenzoyl)-L-Orn-L-Pro-Gly
1.14.99.29 0.2
-
 IC50 0.2 mM Rattus norvegicus N1-acetyl-N4-(2,3-dihydroxybenzoyl)-L-Orn-L-Pro-Gly
1.14.99.29 0.5
-
 IC50 0.5 mM Rattus norvegicus ethyl 3,4-dihydroxybenzoate
1.14.99.29 1.6
-
 IC50 1.6 mM Rattus norvegicus methyl 2,3-dihydroxybenzoate