BRENDA - Enzyme Database show

Properties of a particulate squalene epoxidase from Candida albicans

Ryder, N.S.; Dupont, M.C.; Biochim. Biophys. Acta 794, 466-471 (1984)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.14.14.17
2-heptyl-4-hydroxyquinoline N-oxide
0.1 mM, 236% stimulation
Candida albicans
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.14.17
4-hydroxymercuribenzoate
1 mM, 35% inhibition
Candida albicans
1.14.14.17
antimycin A
0.1 mM, 44% inhibition
Candida albicans
1.14.14.17
bovine serum albumin
-
Candida albicans
1.14.14.17
chloromercuriphenylsulfonate
1 mM, 35% inhibition
Candida albicans
1.14.14.17
Cu2+
5 mM, 99% inhibition
Candida albicans
1.14.14.17
deoxycholate
-
Candida albicans
1.14.14.17
N-ethylmaleimide
1 mM, 35% inhibition
Candida albicans
1.14.14.17
rotenone
0.1 mM, 67% inhibition
Candida albicans
1.14.14.17
Triton X-100
-
Candida albicans
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.17
0.05
-
squalene
-
Candida albicans
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.14.17
microsome
-
Candida albicans
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.14.17
Candida albicans
-
-
-
Storage Stability
EC Number
Storage Stability
Organism
1.14.14.17
-20°C, 5 d, 75% loss of activity
Candida albicans
1.14.14.17
stable for several months in liquid nitrogen
Candida albicans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.17
squalene + NAD(P)H + O2
-
438203
Candida albicans
(S)-squalene-2,3-epoxide + NADP+ + H2O
-
438203
Candida albicans
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.17
FAD
required for activity
Candida albicans
1.14.14.17
additional information
FMN cannot replace FAD as cofactor
Candida albicans
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.14.14.17
2-heptyl-4-hydroxyquinoline N-oxide
0.1 mM, 236% stimulation
Candida albicans
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.17
FAD
required for activity
Candida albicans
1.14.14.17
additional information
FMN cannot replace FAD as cofactor
Candida albicans
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.14.17
4-hydroxymercuribenzoate
1 mM, 35% inhibition
Candida albicans
1.14.14.17
antimycin A
0.1 mM, 44% inhibition
Candida albicans
1.14.14.17
bovine serum albumin
-
Candida albicans
1.14.14.17
chloromercuriphenylsulfonate
1 mM, 35% inhibition
Candida albicans
1.14.14.17
Cu2+
5 mM, 99% inhibition
Candida albicans
1.14.14.17
deoxycholate
-
Candida albicans
1.14.14.17
N-ethylmaleimide
1 mM, 35% inhibition
Candida albicans
1.14.14.17
rotenone
0.1 mM, 67% inhibition
Candida albicans
1.14.14.17
Triton X-100
-
Candida albicans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.17
0.05
-
squalene
-
Candida albicans
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.14.17
microsome
-
Candida albicans
-
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.14.14.17
-20°C, 5 d, 75% loss of activity
Candida albicans
1.14.14.17
stable for several months in liquid nitrogen
Candida albicans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.17
squalene + NAD(P)H + O2
-
438203
Candida albicans
(S)-squalene-2,3-epoxide + NADP+ + H2O
-
438203
Candida albicans
?