BRENDA - Enzyme Database show

Purification and partial characterization of squalene epoxidase from rat liver microsomes

Ono, T.; Nakazono, K.; Kosaka, H.; Biochim. Biophys. Acta 709, 84-90 (1982)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.14.14.17
additional information
enzyme is not a cytochrome P-450 enzyme; reconstitution of squalene epoxidase activity by addition of: NADPH-cytochrome P-450 reductase, EC 1.6.2.4, FAD and Triton X-100
Rattus norvegicus
General Stability
EC Number
General Stability
Organism
1.14.14.17
freezing and thawing once, 20% loss of activity
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.17
0.005
-
FAD
-
Rattus norvegicus
1.14.14.17
0.013
-
squalene
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.14.17
microsome
-
Rattus norvegicus
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.14.17
45000
-
sucrose density gradient centrifugation
Rattus norvegicus
1.14.14.17
51000
-
1 * 51000, most of the enzyme behaves as a monomer, SDS-PAGE
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.14.17
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.14.17
-
Rattus norvegicus
Renatured (Commentary)
EC Number
Commentary
Organism
1.14.14.17
reconstitution of purified enzyme with the addition of NADPH-cytochrome P450 reductase, FAD and Triton X-100
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.17
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.14.17
0.00619
-
-
Rattus norvegicus
Storage Stability
EC Number
Storage Stability
Organism
1.14.14.17
-70°C, 20 mM Tris-HCl buffer, pH 7.4, 0.5% Triton X-100, several weeks
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.17
squalene + NAD(P)H + O2
-
438199
Rattus norvegicus
(S)-squalene-2,3-epoxide + NADP+ + H2O
-
438199
Rattus norvegicus
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.14.17
monomer
1 * 51000, most of the enzyme behaves as a monomer, SDS-PAGE
Rattus norvegicus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.14.14.17
0.0055
-
squalene
-
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.14.14.17
additional information
enzyme is not a cytochrome P-450 enzyme; reconstitution of squalene epoxidase activity by addition of: NADPH-cytochrome P-450 reductase, EC 1.6.2.4, FAD and Triton X-100
Rattus norvegicus
General Stability (protein specific)
EC Number
General Stability
Organism
1.14.14.17
freezing and thawing once, 20% loss of activity
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.17
0.005
-
FAD
-
Rattus norvegicus
1.14.14.17
0.013
-
squalene
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.14.17
microsome
-
Rattus norvegicus
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.14.17
45000
-
sucrose density gradient centrifugation
Rattus norvegicus
1.14.14.17
51000
-
1 * 51000, most of the enzyme behaves as a monomer, SDS-PAGE
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.14.17
-
Rattus norvegicus
Renatured (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.14.17
reconstitution of purified enzyme with the addition of NADPH-cytochrome P450 reductase, FAD and Triton X-100
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.17
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.14.17
0.00619
-
-
Rattus norvegicus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.14.14.17
-70°C, 20 mM Tris-HCl buffer, pH 7.4, 0.5% Triton X-100, several weeks
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.17
squalene + NAD(P)H + O2
-
438199
Rattus norvegicus
(S)-squalene-2,3-epoxide + NADP+ + H2O
-
438199
Rattus norvegicus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.14.17
monomer
1 * 51000, most of the enzyme behaves as a monomer, SDS-PAGE
Rattus norvegicus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.14.14.17
0.0055
-
squalene
-
Rattus norvegicus