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Literature summary extracted from
- Purification and partial characterization of squalene epoxidase from rat liver microsomes (1982), Biochim. Biophys. Acta, 709, 84-90.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.17 | additional information | enzyme is not a cytochrome P-450 enzyme | Rattus norvegicus | |
| 1.14.14.17 | additional information | reconstitution of squalene epoxidase activity by addition of: NADPH-cytochrome P-450 reductase, EC 1.6.2.4, FAD and Triton X-100 | Rattus norvegicus |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.14.14.17 | freezing and thawing once, 20% loss of activity | Rattus norvegicus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.17 | 0.005 | - |
FAD | - |
Rattus norvegicus |  |
| 1.14.14.17 | 0.013 | - |
squalene | - |
Rattus norvegicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.14.17 | microsome | - |
Rattus norvegicus | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.17 | 45000 | - |
sucrose density gradient centrifugation | Rattus norvegicus |
| 1.14.14.17 | 51000 | - |
1 * 51000, most of the enzyme behaves as a monomer, SDS-PAGE | Rattus norvegicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.17 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.14.17 | - |
Rattus norvegicus |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 1.14.14.17 | reconstitution of purified enzyme with the addition of NADPH-cytochrome P450 reductase, FAD and Triton X-100 | Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.14.17 | liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.17 | 0.00619 | - |
- |
Rattus norvegicus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.14.14.17 | -70°C, 20 mM Tris-HCl buffer, pH 7.4, 0.5% Triton X-100, several weeks | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.17 | (3S)-2,3-epoxy-2,3-dihydrosqualene + FAD + H2O | - |
Rattus norvegicus | squalene + FADH2 + O2 | - |
r | |
| 1.14.14.17 | squalene + NAD(P)H + O2 | - |
Rattus norvegicus | (S)-squalene-2,3-epoxide + NADP+ + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.14.17 | monomer | 1 * 51000, most of the enzyme behaves as a monomer, SDS-PAGE | Rattus norvegicus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.17 | 0.0055 | - |
squalene | - |
Rattus norvegicus | |
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Release 2023.1 (January 2023)
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