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Literature summary extracted from

  • Yamakura, F.
    Purification, crystallization and properties of iron-containing superoxide dismutase from Pseudomonas ovalis (1976), Biochim. Biophys. Acta, 422, 280-294.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.15.1.1 8-Hydroxyquinoline-5-sulfonate Fe-SOD, slightly stimulating, depending on the assay method Pseudomonas putida
1.15.1.1 o-phenanthroline Fe-SOD, slightly stimulating Pseudomonas putida
1.15.1.1 o-phenanthroline depending on assay method Pseudomonas putida

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.15.1.1 Fe-SOD, dialysis against 55% saturated ammonium sulfate solution, pH 4.5, 1 week at 2°C under reduced pressure Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.15.1.1 EDTA no inhibition Pseudomonas putida
1.15.1.1 H2O2 Fe-SOD Pseudomonas putida
1.15.1.1 additional information no inhibition by N-ethylmaleide Pseudomonas putida
1.15.1.1 o-phenanthroline depending on assay method; Fe-SOD Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Iron 1.0-1.45 mol per mol of enzyme Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.15.1.1 19500
-
 2 * 19500, Fe-SOD, SDS-PAGE Pseudomonas putida
1.15.1.1 40000
-
 Fe-SOD, gel filtration, sedimentation analysis Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Pseudomonas putida
-
 Fe-SOD
-
1.15.1.1 Pseudomonas putida Fe-SOD
-
 Fe-SOD
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1 Fe-SOD Pseudomonas putida

Reaction

EC Number Reaction Comment Organism Reaction ID
1.15.1.1 2 superoxide + 2 H+ = O2 + H2O2 amino acid composition, comparison Pseudomonas putida
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.15.1.1 additional information
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.1 O2- + H+
-
 Pseudomonas putida O2 + H2O2
-
 ?
1.15.1.1 O2- + H+
-
 Pseudomonas putida Fe-SOD O2 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.15.1.1 dimer 2 * 19500, Fe-SOD, SDS-PAGE Pseudomonas putida