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Literature summary extracted from

  • Anspach, F.B.; Hueckel, M.; Brunke, M.; Schuette, H.; Deckwer, W.D.
    Immobilization of mercuric reductase from a Pseudomonas putida strain on different activated carriers (1994), Appl. Biochem. Biotechnol., 44, 135-150.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.16.1.1 environmental protection application of the immobilized mercuric reductase for continuous treatment of Hg(II)-containing water in a fixed bed reactor Pseudomonas putida

General Stability

EC Number General Stability Organism
1.16.1.1 operational stability: after 1.5 h decline of activity up to 20% Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.16.1.1 additional information
-
 additional information
-
 Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.16.1.1 Pseudomonas putida
-
 KT2442::mer-73
-
1.16.1.1 Pseudomonas putida KT2442::mer-73
-
 KT2442::mer-73
-

Storage Stability

EC Number Storage Stability Organism
1.16.1.1 4°C, 50 mM potassium phosphate buffer, pH 7.2, 0.5 mM EDTA, 1% 2-mercaptoethanol, half-life of soluble enzyme is 3 weeks, immobilized enzyme shows large decline at the beginning and almost no further decrease of activity after 3 weeks Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.16.1.1 Hg2+ + NADPH
-
 Pseudomonas putida Hg + NADP+ + H+
-
 ?
1.16.1.1 Hg2+ + NADPH
-
 Pseudomonas putida KT2442::mer-73 Hg + NADP+ + H+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.16.1.1 NADPH
-
 Pseudomonas putida