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Literature summary extracted from

  • Berglund, O.
    Ribonucleoside diphosphate reductase induced by bacteriophage T4. II. Allosteric regulation of substrate sepecificity and catalytic activity (1972), J. Biol. Chem., 247, 7276-7281.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.4.1 dATP stimulation of CDP and UDP reduction Tequatrovirus T4
1.17.4.1 dCTP stimulation of CDP reduction Tequatrovirus T4
1.17.4.1 dGTP stimulation of ADP reduction Tequatrovirus T4
1.17.4.1 dTTP stimulation of GDP reduction Tequatrovirus T4
1.17.4.1 E. coli thioredoxin reductase absolute requirement Tequatrovirus T4
1.17.4.1 E. coli thioredoxin reductase enzyme induced in Escherichia coli by infection with bacteriophage T4 Tequatrovirus T4

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.4.1 dATP inhibition of ADP reduction; inhibition of CDP reduction; inhibition of GDP reduction; inhibition of UDP reduction Tequatrovirus T4
1.17.4.1 dGTP inhibition of ATP- and dATP stimulated CDP reduction Tequatrovirus T4

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.4.1 additional information
-
 additional information effect of different nucleoside triphosphates on Km Tequatrovirus T4
1.17.4.1 0.04
-
 GDP in the presence of 0.4 mM dTTP as effector Tequatrovirus T4
1.17.4.1 0.043
-
 CDP in the presence of 0.002 mM dATP as effector Tequatrovirus T4
1.17.4.1 0.048
-
 ADP in the presence of 0.007 mM dGTP as effector Tequatrovirus T4
1.17.4.1 0.056
-
 CDP in the presence of 0.5 mM ATP as effector Tequatrovirus T4
1.17.4.1 0.057
-
 CDP in the presence of 0.02 mM dTTP as effector Tequatrovirus T4
1.17.4.1 0.1
-
 UDP in the presence of 0.3 mM ATP as effector Tequatrovirus T4
1.17.4.1 0.3
-
 ADP
-
 Tequatrovirus T4
1.17.4.1 0.31
-
 CDP
-
 Tequatrovirus T4
1.17.4.1 0.37
-
 GDP
-
 Tequatrovirus T4
1.17.4.1 1.2
-
 UDP
-
 Tequatrovirus T4

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.1 Tequatrovirus T4
-
 enzyme induced in Escherichia coli by infection with bacteriophage T4
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.1 ADP + thioredoxin
-
 Tequatrovirus T4 2'-dADP + thioredoxin disulfide + H2O
-
 r
1.17.4.1 CDP + thioredoxin
-
 Tequatrovirus T4 dCDP + thioredoxin disulfide + H2O
-
 r
1.17.4.1 CDP + thioredoxin
-
 Tequatrovirus T4 2'-dCDP + thioredoxin disulfide + H2O
-
 r
1.17.4.1 GDP + thioredoxin
-
 Tequatrovirus T4 2'-dGDP + thioredoxin disulfide + H2O
-
 r
1.17.4.1 ribonucleoside diphosphate + reduced thioredoxin
-
 Tequatrovirus T4 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
 ir
1.17.4.1 UDP + reduced thioredoxin
-
 Tequatrovirus T4 2'-deoxy-UDP + oxidized thioredoxin + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.4.1 T4 thioredoxin enzyme induced in E. coli by infection with bacteriophage T4 Tequatrovirus T4

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.17.4.1 0.0002
-
 dATP in the presence of 0.15 mM dGTP Tequatrovirus T4
1.17.4.1 0.0004
-
 dGTP with 0.001 mM dATP as positive effector Tequatrovirus T4
1.17.4.1 0.0004
-
 dATP in the presence of 0.55 mM dTTP Tequatrovirus T4
1.17.4.1 0.0005
-
 dGTP with 0.02 mM dATP as positive effector Tequatrovirus T4
1.17.4.1 0.0005
-
 dATP in the presence of 0.005 mM dGTP Tequatrovirus T4
1.17.4.1 0.0006
-
 dATP in the presence of 0.05 mM dTTP Tequatrovirus T4
1.17.4.1 0.0009
-
 dGTP with 0.17 mM ATP as positive effector Tequatrovirus T4
1.17.4.1 0.0018
-
 dGTP with 1.7 mM ATP as positive effector Tequatrovirus T4