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Literature summary extracted from

  • Kucera, R.; Paulus, H.
    Studies on ribonucleoside-diphosphate reductase in permeable animal cells. II. Catalytic and regulatory properties of the enzyme in mouse L cells (1982), Arch. Biochem. Biophys., 214, 114-123.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.4.1 adenyl-5'-yl-imidodiphosphate maximal activation of CDP reduction at 4 mM Mus musculus
1.17.4.1 ATP reduction of CDP is dependent on ATP or adenyl-5'-yl iminodiphosphate Mus musculus
1.17.4.1 dihydrolipoic acid slight stimulation Mus musculus
1.17.4.1 dithiothreitol slight activation Mus musculus
1.17.4.1 dTTP stimulation of GDP reduction Mus musculus
1.17.4.1 EDTA reversible stimulation of GDP reduction, irreversible inhibition of CDP reduction Mus musculus
1.17.4.1 additional information enzyme of cells first treated with 2,6-dichlorophenolindophenol has a complete dependence on NADPH which can also be met by dithiothreitol or dihydrolipoic acid Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.4.1 ATP 4 mM, 50% inhibition of GDP reduction in the presence of dTTP Mus musculus
1.17.4.1 bathophenanthroline sulfonate 5 mM, almost complete inhibition of CDP and GDP reduction Mus musculus
1.17.4.1 dATP 0.0033 mM, 50% inhibition of CDP reduction, 0.0036 mM, 50% inhibition of GDP reduction; inhibition of CDP reduction Mus musculus
1.17.4.1 dCTP 1 mM, 50% inhibition of CDP reduction Mus musculus
1.17.4.1 dGTP 0.08 mM, 50% inhibition of CDP reduction, 0.19 mM, 50% inhibition of GDP reduction; inhibition of CDP reduction Mus musculus
1.17.4.1 dTTP 0.2 mM, 50% inhibition of CDP reduction Mus musculus
1.17.4.1 EDTA reversible stimulation of GDP reduction, irreversible inhibition of CDP reduction Mus musculus
1.17.4.1 Hydroxyurea 1 mM, 98 and 81% inhibition of CDP and GDP reduction respectively Mus musculus
1.17.4.1 Mg2+ 1-5 mM, 10-20% inhibition of GDP reduction; inhibition of CDP reduction Mus musculus
1.17.4.1 sodium arsenite 0.025 mM, almost complete inhibition of CDP reduction, 86% inhibition of GDP reduction Mus musculus
1.17.4.1 Thenoyltrifluoroacetone 5 mM, almost complete inhibition of CDP and GDP reduction Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.4.1 0.05
-
CDP
-
Mus musculus
1.17.4.1 0.24
-
GDP
-
Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.1 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.4.1 Novikoff ascites tumor cells, partial Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.1 ADP + reduced thioredoxin
-
Mus musculus 2'-deoxy-ADP + oxidized thioredoxin + H2O
-
ir
1.17.4.1 CDP + reduced thioredoxin
-
Mus musculus 2'-deoxy-CDP + oxidized thioredoxin + H2O
-
ir
1.17.4.1 GDP + reduced thioredoxin
-
Mus musculus 2'-deoxy-GDP + oxidized thioredoxin + H2O
-
ir
1.17.4.1 ribonucleoside diphosphate + reduced thioredoxin
-
Mus musculus 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
ir
1.17.4.1 UDP + reduced thioredoxin
-
Mus musculus 2'-deoxy-UDP + oxidized thioredoxin + H2O
-
ir

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.4.1 NADPH slight stimulation Mus musculus