EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.17.4.2 | dATP | stimulation of CTP reduction | Lactobacillus leichmannii | |
1.17.4.2 | dCTP | stimulation of UTP reduction | Lactobacillus leichmannii | |
1.17.4.2 | dGTP | stimulation of ATP reduction | Lactobacillus leichmannii | |
1.17.4.2 | dTTP | stimulation of GTP reduction | Lactobacillus leichmannii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.17.4.2 | 69300 | - |
enzyme in 6 M guanidine-HCl, 0.1 M dithiothreitol, equilibrium ultracentrifugation | Lactobacillus leichmannii |
1.17.4.2 | 72500 | - |
native enzyme in 0.1 M glycine buffer, pH 9.1, equilibrium ultracentrifugation | Lactobacillus leichmannii |
1.17.4.2 | 72600 | - |
maleated enzyme in 0.05 M phosphate buffer, equilibrium ultracentrifugation | Lactobacillus leichmannii |
1.17.4.2 | 74500 | - |
enzyme in 6 M guanidine-HCl, 0.1 M dithiothreitol, heated for 20 min at 60°C, equilibrium ultracentrifugation | Lactobacillus leichmannii |
1.17.4.2 | 75300 | - |
native enzyme in 0.05 M phosphate buffer, pH 7.0, sedimentation equilibrium method | Lactobacillus leichmannii |
1.17.4.2 | 76000 | - |
gel filtration | Lactobacillus leichmannii |
1.17.4.2 | 78400 | - |
SDS-PAGE | Lactobacillus leichmannii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.17.4.2 | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | Lactobacillus leichmannii | - |
ribonucleoside triphosphate + reduced thioredoxin | - |
r | |
1.17.4.2 | ribonucleoside triphosphate + reduced thioredoxin + H2O | Lactobacillus leichmannii | - |
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.17.4.2 | Lactobacillus leichmannii | - |
strain ATCC 7630 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.17.4.2 | - |
Lactobacillus leichmannii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.17.4.2 | 16 | - |
- |
Lactobacillus leichmannii |
EC Number | Storage Stability | Organism |
---|---|---|
1.17.4.2 | -65°C, no detectable loss of enzyme has been observed during several years of storage of packed bacteria | Lactobacillus leichmannii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.17.4.2 | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
Lactobacillus leichmannii | ribonucleoside triphosphate + reduced thioredoxin | - |
r | |
1.17.4.2 | ribonucleoside triphosphate + reduced thioredoxin | - |
Lactobacillus leichmannii | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
1.17.4.2 | ribonucleoside triphosphate + reduced thioredoxin + H2O | - |
Lactobacillus leichmannii | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.17.4.2 | monomer | 1 * 78400, SDS-PAGE | Lactobacillus leichmannii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.17.4.2 | coenzyme B12 | - |
Lactobacillus leichmannii |