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Literature summary extracted from

  • Keat, M.J.; Hopper, D.J.
    p-Cresol and 3,5-xylenol methylhydroxylases in Pseudomonas putida N.C.I.B. 9896 (1978), Biochem. J., 175, 649-658.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.9.1 0.0036
-
4-Cresol hydroxylase B Pseudomonas putida
1.17.9.1 0.015
-
4-Cresol hydroxylase B, 4-hydroxybenzyl alcohol Pseudomonas putida
1.17.9.1 0.016
-
4-Cresol hydroxylase A Pseudomonas putida
1.17.9.1 0.027
-
4-Hydroxybenzyl alcohol hydroxylase A Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.9.1 56000
-
2 * 56000, hydroxylase B, SDS-PAGE after incubation with mercaptoethanol Pseudomonas putida
1.17.9.1 58000
-
2 * 58000, hydroxylase A, SDS-PAGE after incubation with mercaptoethanol Pseudomonas putida
1.17.9.1 99000
-
hydroxylase B, equilibrium sedimentation Pseudomonas putida
1.17.9.1 100000
-
hydroxylase B, gel filtration Pseudomonas putida
1.17.9.1 108000
-
hydroxylase A, gel filtration Pseudomonas putida
1.17.9.1 114000
-
hydroxylase A, equilibrium sedimentation Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.17.9.1 Pseudomonas putida
-
-
-
1.17.9.1 Pseudomonas putida
-
2 enzyme forms with various MW and Km are formed during growth on 3,5-xylenol - hydroxylase A - and 4-cresol - hydroxylase B
-
1.17.9.1 Pseudomonas putida NCIB 9869
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.9.1
-
Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.9.1 additional information
-
-
Pseudomonas putida
1.17.9.1 6.78
-
hydroxylase B Pseudomonas putida
1.17.9.1 9.95
-
hydroxylase A Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.9.1 2,4-xylenol + acceptor + H2O i.e. 2,4-dimethylphenol Pseudomonas putida 3-methyl-4-hydroxybenzaldehyde + reduced acceptor
-
?
1.17.9.1 3,4-dimethylphenol + acceptor + H2O
-
Pseudomonas putida 2-methyl-4-hydroxybenzaldehyde + reduced acceptor
-
?
1.17.9.1 4-cresol + acceptor + H2O
-
Pseudomonas putida 4-hydroxybenzaldehyde + reduced acceptor
-
?
1.17.9.1 4-cresol + acceptor + H2O
-
Pseudomonas putida NCIB 9869 4-hydroxybenzaldehyde + reduced acceptor
-
?
1.17.9.1 4-ethylphenol + acceptor + H2O
-
Pseudomonas putida 1-(4'-hydroxyphenyl)ethanol + reduced acceptor
-
?
1.17.9.1 4-ethylphenol + acceptor + H2O
-
Pseudomonas putida NCIB 9869 1-(4'-hydroxyphenyl)ethanol + reduced acceptor
-
?
1.17.9.1 4-hydroxybenzyl alcohol + acceptor
-
Pseudomonas putida 4-hydroxybenzaldehyde + reduced acceptor
-
?
1.17.9.1 4-hydroxybenzyl alcohol + acceptor
-
Pseudomonas putida NCIB 9869 4-hydroxybenzaldehyde + reduced acceptor
-
?
1.17.9.1 additional information no activity with 2,3-xylenol and 2,5-xylenol Pseudomonas putida ?
-
?
1.17.9.1 additional information no activity with 2-cresol Pseudomonas putida ?
-
?
1.17.9.1 additional information no activity with 2,3-xylenol and 2,5-xylenol Pseudomonas putida NCIB 9869 ?
-
?
1.17.9.1 additional information no activity with 2-cresol Pseudomonas putida NCIB 9869 ?
-
?

Subunits

EC Number Subunits Comment Organism
1.17.9.1 dimer 2 * 58000, hydroxylase A, SDS-PAGE after incubation with mercaptoethanol Pseudomonas putida
1.17.9.1 dimer 2 * 56000, hydroxylase B, SDS-PAGE after incubation with mercaptoethanol Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.17.9.1 p-cresol methylhydroxylase A 2 enzyme forms with various MW and Km are formed during growth on 3,5-xylenol - hydroxylase A - and 4-cresol - hydroxylase B Pseudomonas putida
1.17.9.1 p-cresol methylhydroxylase B 2 enzyme forms with various MW and Km are formed during growth on 3,5-xylenol - formation of hydroxylase A - and on 4-cresol - formation of hydroxylase B Pseudomonas putida