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Literature summary extracted from
- Investigations on microbial sulfur respiration (1988), Arch. Microbiol., 150, 72-77.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.12.98.4 | cysteamine | increase of activity | Sulfurospirillum deleyianum |  |
| 1.12.98.4 | cysteine | increase of activity | Sulfurospirillum deleyianum | |
| 1.12.98.4 | GSH | reduced form of glutathione, increase of activity | Sulfurospirillum deleyianum | |
| 1.12.98.4 | additional information | all organisms except Spirillum sp. require an external hydrogenase for assay | Sulfurospirillum deleyianum |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.12.98.4 | 2-(n-heptyl)-4-hydroxyquinoline N-oxide | strong in manometric assay | Sulfurospirillum deleyianum | |
| 1.12.98.4 | cystamine | - |
Sulfurospirillum deleyianum | |
| 1.12.98.4 | cystine | - |
Sulfurospirillum deleyianum | |
| 1.12.98.4 | GSSG | 90% loss of activity | Sulfurospirillum deleyianum | |
| 1.12.98.4 | additional information | no inhibition by rotenone, dicoumarol, antimycin A, NaN3, sodium dithiocarbamate | Sulfurospirillum deleyianum | |
| 1.12.98.4 | p-chloromercuribenzene sulfonic acid | - |
Sulfurospirillum deleyianum | |
| 1.12.98.4 | Quinacrine | strong | Sulfurospirillum deleyianum | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.12.98.4 | cytoplasm | - |
Desulfomicrobium baculatum | 5737 | - |
| 1.12.98.4 | cytoplasm | - |
Desulfuromonas acetoxidans | 5737 | - |
| 1.12.98.4 | cytoplasm | - |
Desulfuromonas acetexigens | 5737 | - |
| 1.12.98.4 | cytoplasm | - |
Desulfuromusa succinoxidans | 5737 | - |
| 1.12.98.4 | cytoplasm | no activity | Sulfurospirillum deleyianum | 5737 | - |
| 1.12.98.4 | membrane | - |
Desulfomicrobium baculatum | 16020 | - |
| 1.12.98.4 | membrane | - |
Desulfuromonas acetoxidans | 16020 | - |
| 1.12.98.4 | membrane | - |
Sulfurospirillum deleyianum | 16020 | - |
| 1.12.98.4 | membrane | - |
Desulfuromonas acetexigens | 16020 | - |
| 1.12.98.4 | membrane | - |
Desulfuromusa succinoxidans | 16020 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.12.98.4 | sulfur | Sulfurospirillum deleyianum | constitutive membrane-bound enzyme | H2S | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.12.98.4 | Desulfomicrobium baculatum | - |
- |
- |
| 1.12.98.4 | Desulfomicrobium baculatum 9974 | - |
- |
- |
| 1.12.98.4 | Desulfuromonas acetexigens | - |
- |
- |
| 1.12.98.4 | Desulfuromonas acetexigens 2873 | - |
- |
- |
| 1.12.98.4 | Desulfuromonas acetoxidans | - |
- |
- |
| 1.12.98.4 | Desulfuromonas acetoxidans 5071 | - |
- |
- |
| 1.12.98.4 | Desulfuromusa succinoxidans | - |
strain Gö20 | - |
| 1.12.98.4 | Desulfuromusa succinoxidans Go20 | - |
strain Gö20 | - |
| 1.12.98.4 | Sulfurospirillum deleyianum | - |
formerly called Spirillum sp. strain 5175, enzyme formed during growth with sulfur, DSM 6946 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.12.98.4 | partial, solubilized with Triton X-100 | Sulfurospirillum deleyianum |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.12.98.4 | H2 + (sulfide)n = hydrogen sulfide + (sulfide)n-1 | sulfur can be reduced with hydrogen as donor in the presence of hydrogenase | Sulfurospirillum deleyianum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.12.98.4 | 0.026 | - |
crude extract | Desulfuromonas acetoxidans |
| 1.12.98.4 | 0.04 | - |
crude extract | Desulfuromusa succinoxidans |
| 1.12.98.4 | 0.043 | - |
crude extract | Desulfomicrobium baculatum |
| 1.12.98.4 | 0.07 | - |
crude extract | Desulfuromonas acetexigens |
| 1.12.98.4 | 0.27 | - |
crude extract | Sulfurospirillum deleyianum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.12.98.4 | additional information | sulfur can be reduced by photochemical reduction in the presence of phenosafranin | Sulfurospirillum deleyianum | ? | - |
? | |
| 1.12.98.4 | additional information | no substrates are sulfite, thiosulfate, tetrathionate | Sulfurospirillum deleyianum | ? | - |
? | |
| 1.12.98.4 | organic trisulfides + electron donor | i.e. (R-S-S-S-R)2, R: CH2CH2NH2 | Sulfurospirillum deleyianum | H2S + oxidized electron donor | - |
? | |
| 1.12.98.4 | organic trisulfides + electron donor | R: CH2CH(COOH)NH2, CH2CH2SO3Na or CH2CH2COONa, reduced at a rate comparable to elemental sulfur | Sulfurospirillum deleyianum | H2S + oxidized electron donor | - |
? | |
| 1.12.98.4 | polysulfide + electron donor | comparable to colloidal sulfur | Sulfurospirillum deleyianum | H2S + oxidized electron donor | - |
? | |
| 1.12.98.4 | sulfur | constitutive membrane-bound enzyme | Sulfurospirillum deleyianum | H2S | - |
? | |
| 1.12.98.4 | sulfur + electron donor | - |
Desulfomicrobium baculatum | H2S + oxidized electron donor | - |
? | |
| 1.12.98.4 | sulfur + electron donor | - |
Desulfuromonas acetoxidans | H2S + oxidized electron donor | - |
? | |
| 1.12.98.4 | sulfur + electron donor | - |
Desulfuromonas acetexigens | H2S + oxidized electron donor | - |
? | |
| 1.12.98.4 | sulfur + electron donor | - |
Desulfuromusa succinoxidans | H2S + oxidized electron donor | - |
? | |
| 1.12.98.4 | sulfur + electron donor | elemental: best results with colloidal Janek sulfur, hydrophilic sulfur yields generally lower activities | Sulfurospirillum deleyianum | H2S + oxidized electron donor | - |
? | |
| 1.12.98.4 | sulfur + electron donor | - |
Desulfuromonas acetexigens 2873 | H2S + oxidized electron donor | - |
? | |
| 1.12.98.4 | sulfur + electron donor | - |
Desulfomicrobium baculatum 9974 | H2S + oxidized electron donor | - |
? | |
| 1.12.98.4 | sulfur + electron donor | - |
Desulfuromonas acetoxidans 5071 | H2S + oxidized electron donor | - |
? | |
| 1.12.98.4 | sulfur + electron donor | - |
Desulfuromusa succinoxidans Go20 | H2S + oxidized electron donor | - |
? | |
| 1.12.98.4 | tetrasulfide + electron donor | comparable to colloidal sulfur | Sulfurospirillum deleyianum | H2S + oxidized electron donor | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.12.98.4 | 8.7 | 8.9 | - |
Sulfurospirillum deleyianum |
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