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Literature summary extracted from

  • Bamforth, C.W.; Large, P.J.
    Solubilization, partial purification and properties of N-methylglutamate dehydrogenase from Pseudomonas aminovorans (1977), Biochem. J., 161, 357-370.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.99.5 2-oxoglutarate
-
Aminobacter aminovorans
1.5.99.5 Cu2+ total inhibition at 1 mM Aminobacter aminovorans
1.5.99.5 formaldehyde
-
Aminobacter aminovorans
1.5.99.5 iodoacetamide
-
Aminobacter aminovorans
1.5.99.5 p-chloromercuribenzoate
-
Aminobacter aminovorans
1.5.99.5 Triton X-100
-
Aminobacter aminovorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.99.5 0.025
-
N-Methyl-L-glutamate
-
Aminobacter aminovorans
1.5.99.5 0.21
-
2,6-dichlorophenolindophenol
-
Aminobacter aminovorans
1.5.99.5 0.46
-
N-methyl DL-aspartate
-
Aminobacter aminovorans
1.5.99.5 25
-
N-methyl L-alanine
-
Aminobacter aminovorans
1.5.99.5 33
-
N-methyl D-alanine
-
Aminobacter aminovorans
1.5.99.5 87
-
N-methyl DL-alanine
-
Aminobacter aminovorans
1.5.99.5 90.9
-
sarcosine
-
Aminobacter aminovorans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.99.5 membrane
-
Aminobacter aminovorans 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.99.5 Co2+ 1 mM stimulates activity by 33% Aminobacter aminovorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.99.5 N-methyl-L-glutamate + acceptor + H2O Aminobacter aminovorans
-
L-glutamate + formaldehyde + reduced acceptor
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.5.99.5 Aminobacter aminovorans
-
-
-
1.5.99.5 Aminobacter aminovorans AM1
-
-
-
1.5.99.5 Aminobacter aminovorans N.C.I.B. 9039
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.99.5 partial Aminobacter aminovorans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.99.5 0.00033
-
crude extract, grown on trimethylamine N-oxide Aminobacter aminovorans
1.5.99.5 0.0018
-
crude extract, grown on trimethylamine Aminobacter aminovorans
1.5.99.5 0.0025
-
crude extract, grown on methylamine Aminobacter aminovorans
1.5.99.5 0.00364
-
crude extract, grown on dimethylamine Aminobacter aminovorans
1.5.99.5 0.0554
-
-
Aminobacter aminovorans

Storage Stability

EC Number Storage Stability Organism
1.5.99.5 -15°C, stable to repeated freezing and thawing and storage at a protein concentration of 3 mg/ml Aminobacter aminovorans
1.5.99.5 -196°C, survives cooling with subsequent thawing Aminobacter aminovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.99.5 N-methyl D-alanine + 2,6-dichlorophenolindophenol + H2O
-
Aminobacter aminovorans D-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
?
1.5.99.5 N-methyl DL-alanine + 2,6-dichlorophenolindophenol + H2O
-
Aminobacter aminovorans DL-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
?
1.5.99.5 N-methyl DL-aspartate + 2,6-dichlorophenolindophenol + H2O
-
Aminobacter aminovorans DL-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
?
1.5.99.5 N-methyl DL-aspartate + 2,6-dichlorophenolindophenol + H2O
-
Aminobacter aminovorans AM1 DL-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
?
1.5.99.5 N-methyl DL-aspartate + 2,6-dichlorophenolindophenol + H2O
-
Aminobacter aminovorans N.C.I.B. 9039 DL-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
?
1.5.99.5 N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O
-
Aminobacter aminovorans L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
?
1.5.99.5 N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O
-
Aminobacter aminovorans AM1 L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
?
1.5.99.5 N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O
-
Aminobacter aminovorans N.C.I.B. 9039 L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
?
1.5.99.5 N-methyl L-aspartate + 2,6-dichlorophenolindophenol + H2O
-
Aminobacter aminovorans L-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
?
1.5.99.5 N-methyl L-aspartate + 2,6-dichlorophenolindophenol + H2O
-
Aminobacter aminovorans AM1 L-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
?
1.5.99.5 N-methyl L-aspartate + 2,6-dichlorophenolindophenol + H2O
-
Aminobacter aminovorans N.C.I.B. 9039 L-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
?
1.5.99.5 N-methyl-L-glutamate + acceptor + H2O
-
Aminobacter aminovorans L-glutamate + formaldehyde + reduced acceptor
-
?
1.5.99.5 N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
Aminobacter aminovorans L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
?
1.5.99.5 N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
Aminobacter aminovorans AM1 L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
?
1.5.99.5 N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
Aminobacter aminovorans N.C.I.B. 9039 L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
?
1.5.99.5 N-methylglutamate + phenazine methosulfate + H2O
-
Aminobacter aminovorans L-glutamate + formaldehyde + reduced phenazine methosulfate
-
?
1.5.99.5 N-methylglutamate + phenazine methosulfate + H2O
-
Aminobacter aminovorans AM1 L-glutamate + formaldehyde + reduced phenazine methosulfate
-
?
1.5.99.5 N-methylglutamate + phenazine methosulfate + H2O
-
Aminobacter aminovorans N.C.I.B. 9039 L-glutamate + formaldehyde + reduced phenazine methosulfate
-
?
1.5.99.5 sarcosine + 2,6-dichlorophenolindophenol + H2O
-
Aminobacter aminovorans aminoacetate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.99.5 30 40 heat-labile with half-lives of 35 min at 30°C and 5 min at 40°C Aminobacter aminovorans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.99.5 5.5 9
-
Aminobacter aminovorans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.5.99.5 7
-
acid labile, stable at neutrality and pH values above 7.0 Aminobacter aminovorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.99.5 cytochrome b
-
Aminobacter aminovorans