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Literature summary extracted from

  • Arata, H.; Shimizu, M.; Takamiya, K.
    Purification and properties of trimethylamine N-oxide reductase from aerobic photosynthetic bacterium Roseobacter denitrificans (1992), J. Biochem., 112, 470-475.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.7.2.3 activity is easily lost on dialysis Roseobacter denitrificans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.2.3 0.006
-
 Trimethylamine N-oxide
-
 Roseobacter denitrificans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.3 Molybdenum
-
 Roseobacter denitrificans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.2.3 90000
-
 2 * 90000, SDS-PAGE Roseobacter denitrificans
1.7.2.3 172000
-
 gel filtration Roseobacter denitrificans

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.3 Roseobacter denitrificans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.3
-
 Roseobacter denitrificans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.2.3 49.5
-
-
 Roseobacter denitrificans

Storage Stability

EC Number Storage Stability Organism
1.7.2.3 -80°C stable for several months Roseobacter denitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.3 bromate + (ferrocytochrome c)-subunit
-
 Roseobacter denitrificans ? + (ferricytochrome c)-subunit
-
 ?
1.7.2.3 hydroxylamine + (ferrocytochrome c)-subunit
-
 Roseobacter denitrificans ? + (ferricytochrome c)-subunit
-
 ?
1.7.2.3 nicotinic acid N-oxide + (ferrocytochrome c)-subunit + H+
-
 Roseobacter denitrificans nicotinic acid + (ferricytochrome c)-subunit + H2O
-
 ?
1.7.2.3 picoline N-oxide + (ferrocytochrome c)-subunit + H+
-
 Roseobacter denitrificans picoline + (ferricytochrome c)-subunit + H2O
-
 ?
1.7.2.3 trimethylamine N-oxide + (ferrocytochrome c)-subunit + H+ cytochrome c2, cytochrome c552 and methylviologen can serve as electron donors Roseobacter denitrificans trimethylamine + (ferricytochrome c)-subunit + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.2.3 dimer 2 * 90000, SDS-PAGE Roseobacter denitrificans

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.2.3 heme c
-
 Roseobacter denitrificans