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Literature summary extracted from

  • Trueman, R.J.; Tiku, P.E.; Caddick, M.X.; Cossins, A.R.
    Thermal thresholds of lipid restructuring and DELTA9-desaturase expression in the liver of carp (Cyprinus carpio L.) (2000), J. Exp. Biol., 203, 641-650.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.19.1 cytochrome b5 reductase
-
 Rattus norvegicus
1.14.19.1 cytochrome b5 reductase NADH-dependent Cyprinus carpio

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.1 endoplasmic reticulum
-
 Rattus norvegicus 5783
-
1.14.19.1 endoplasmic reticulum
-
 Cyprinus carpio 5783
-
1.14.19.1 microsome
-
 Rattus norvegicus
-
-
1.14.19.1 microsome
-
 Cyprinus carpio
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.19.1 33000
-
-
 Cyprinus carpio

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.1 Cyprinus carpio
-
 L.
-
1.14.19.1 Cyprinus carpio L.
-
 L.
-
1.14.19.1 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.19.1
-
 Cyprinus carpio

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.19.1 stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = oleoyl-CoA + 2 ferricytochrome b5 + 2 H2O presence of two desaturase genes, isomers CDS1 and CDS2. Cooling treatment of carp causes significant changes to the activity of the DELTA9-desaturase. Modest cooling of carp shows an increase in acticity without any increase in the amount of desaturase protein, suggesting the activation of the pre-existing inactive latent enzyme, possibly by a post-translational mechanism Cyprinus carpio
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.19.1 liver
-
 Rattus norvegicus
-
1.14.19.1 liver
-
 Cyprinus carpio
-

Storage Stability

EC Number Storage Stability Organism
1.14.19.1 -80°C, saline solution, 150 mM NaCl, 0.1 mM EDTA, 20 mM HEPES, pH 7.4 Cyprinus carpio

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.1 palmitoyl-CoA + AH2 + O2
-
 Cyprinus carpio palmitoleoyl-CoA + A
-
 ?
1.14.19.1 palmitoyl-CoA + AH2 + O2
-
 Cyprinus carpio L. palmitoleoyl-CoA + A
-
 ?
1.14.19.1 stearoyl-CoA + AH2 + O2
-
 Cyprinus carpio oleoyl-CoA + A + H2O
-
 ?
1.14.19.1 stearoyl-CoA + AH2 + O2
-
 Cyprinus carpio L. oleoyl-CoA + A + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.1 cytochrome b5
-
 Rattus norvegicus
1.14.19.1 cytochrome b5
-
 Cyprinus carpio
1.14.19.1 NADH
-
 Rattus norvegicus
1.14.19.1 NADH
-
 Cyprinus carpio