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Literature summary extracted from

  • Strittmatter, P.; Enoch, H.G.
    Purification of stearyl-CoA desaturase from liver (1978), Methods Enzymol., 52, 188-193.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.14.19.1 relatively labil Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.19.1 CN- at high concentrations Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.19.1 additional information
-
 additional information substrates with 14-19 carbon fatty acyl chains show similar Km values of 0.0045-0.005 mM Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.1 microsome
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.19.1 Fe2+ contains one atom of non-heme iron per enzyme molecule, catalytic function Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.1 stearoyl-CoA + AH2 + O2 Rattus norvegicus
-
 oleoyl-CoA + A + H2O
-
 ?
1.14.19.1 stearoyl-CoA + AH2 + O2 Rattus norvegicus Long-Evans
-
 oleoyl-CoA + A + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.1 Rattus norvegicus
-
 Long-Evans
-
1.14.19.1 Rattus norvegicus Long-Evans
-
 Long-Evans
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.14.19.1 additional information no detectable carbohydrate Rattus norvegicus
1.14.19.1 phospholipoprotein 4-12 mol of phospholipid per mol of polypeptide Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.19.1 different fractions by sonication and centrifugation, ion-exchange Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.19.1 stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = oleoyl-CoA + 2 ferricytochrome b5 + 2 H2O cytochrome b5, cytochrome b5 reductase and lipid and/or detergent are needed for all assays. A cis-hydrogen abstraction mechanism is involved in desaturation Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.19.1 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.19.1 0.325
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.14.19.1 -75°C, more than 95% pure last fraction, 3 months, no loss of activity Rattus norvegicus
1.14.19.1 -75°C, fractions during purification, overnight, 20-50% loss of activity Rattus norvegicus
1.14.19.1 0°C, above 95% pure last fraction, 15-20 hours, 30-50% loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.1 additional information 12-19 carbon fatty acyl chains undergo 9,10 desaturation. Dephospho-, deamino- and (1-N6-etheno)-CoA analogs of stearoyl-CoA are poorer substrates than stearoyl-CoA Rattus norvegicus ?
-
 ?
1.14.19.1 additional information 12-19 carbon fatty acyl chains undergo 9,10 desaturation. Dephospho-, deamino- and (1-N6-etheno)-CoA analogs of stearoyl-CoA are poorer substrates than stearoyl-CoA Rattus norvegicus Long-Evans ?
-
 ?
1.14.19.1 stearoyl-CoA + AH2 + O2
-
 Rattus norvegicus oleoyl-CoA + A + H2O
-
 ?
1.14.19.1 stearoyl-CoA + AH2 + O2
-
 Rattus norvegicus Long-Evans oleoyl-CoA + A + H2O
-
 ?
1.14.19.1 stearoyl-CoA + NADH + O2
-
 Rattus norvegicus oleoyl-CoA + NAD+ + H2O
-
 ?
1.14.19.1 stearoyl-CoA + NADH + O2
-
 Rattus norvegicus Long-Evans oleoyl-CoA + NAD+ + H2O
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.19.1 25
-
-
 Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.19.1 0.35
-
 stearoyl-CoA fatty acyl-CoA substrates with chain length of 14-19 carbon atoms show similar turnover number of about 22 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.1 NADH
-
 Rattus norvegicus