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Literature summary extracted from

  • Jones, D.P.; Gaylor, J.L.
    A non-substrate-binding protein that stimulates microsomal stearyl-CoA desaturase (1981), Methods Enzymol., 71, 258-263.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.19.1 protein non-substrate soluble cytosolic protein stimulates Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.1 microsome
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.19.1 26500
-
 gel filtration Rattus norvegicus
1.14.19.1 27000
-
 1 * 27000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.1 stearoyl-CoA + AH2 + O2 Rattus norvegicus also other fatty acyl-CoA substrates oleoyl-CoA + A + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.1 Rattus norvegicus
-
 Sprague-Dawley, male
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.19.1 ammonium sulfate precipitation, gel filtration, ultra filtration, ion-exchange Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.19.1 stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = oleoyl-CoA + 2 ferricytochrome b5 + 2 H2O multienzyme, insertion of a double bond in 9,10 position Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.19.1 liver
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
1.14.19.1 4°C, microsomes, 24 hours, inhibition of desaturase by the cytosolic fraction rather than stimulation Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.1 stearoyl-CoA + AH2 + O2 also other fatty acyl-CoA substrates Rattus norvegicus oleoyl-CoA + A + H2O
-
 ?
1.14.19.1 stearoyl-CoA + NADH + O2
-
 Rattus norvegicus oleoyl-CoA + NAD+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.19.1 monomer 1 * 27000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.19.1 37
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.1 NADH
-
 Rattus norvegicus