EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.4 | spermidine | in absence of Mg2+ spermine allows a very low level of cytoplasmic enzyme | Euglena gracilis | |
6.1.1.9 | spermine | chloroplastic enzyme: unable to promote aminoacylation in absence of Mg2+. In presence of suboptimal concentrations of Mg2+, spermine can restore maximal activity (not in cytoplasmic enzyme) | Euglena gracilis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.9 | KCl | above 30 mM: inhibits activity of cytoplasmic enzyme more strongly than that of chloroplastic enzyme, 30 mM: enhances activity of the cytoplasmic enzyme, has no effect on the chloroplastic enzyme | Euglena gracilis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.9 | 0.00005 | - |
tRNAVal | of Euglena gracilis | Euglena gracilis | |
6.1.1.9 | 0.00005 | - |
tRNAVal | cytoplasmic enzyme | Euglena gracilis | |
6.1.1.9 | 0.00006 | - |
tRNAVal | of Euglena gracilis, , chloroplastic enzyme | Euglena gracilis | |
6.1.1.9 | 0.00008 | - |
tRNAVal | tRNA, of E. coli, , chloroplasmic enzyme | Euglena gracilis | |
6.1.1.9 | 0.00008 | - |
tRNAVal | tRNA, of yeast, , cytoplasmic enzyme | Euglena gracilis | |
6.1.1.9 | 0.015 | - |
L-valine | chloroplastic enzyme | Euglena gracilis | |
6.1.1.9 | 0.05 | - |
L-valine | cytoplasmic enzyme | Euglena gracilis | |
6.1.1.9 | 0.05 | - |
L-valine | ATP, cytoplasmic enzyme | Euglena gracilis | |
6.1.1.9 | 0.07 | - |
ATP | cytoplasmic enzyme | Euglena gracilis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
6.1.1.4 | chloroplast | - |
Euglena gracilis | 9507 | - |
6.1.1.4 | cytoplasm | - |
Euglena gracilis | 5737 | - |
6.1.1.9 | cytoplasm | - |
Euglena gracilis | 5737 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.4 | Ca2+ | can partially replace Mg2+ in activation (in chloroplastic enzyme, not in cytoplasmic enzyme) | Euglena gracilis | |
6.1.1.4 | Co2+ | can partially replace Mg2+ in activation (in chloroplastic enzyme, not in cytoplasmic enzyme | Euglena gracilis | |
6.1.1.4 | K+ | 30 mM KCl enhance activity of cytoplasmic enzyme, no effect on chloroplastic enzyme, concentrations higher than 30 mM inhibit cytoplasmic enzyme more strongly than chloroplastic enzyme | Euglena gracilis | |
6.1.1.4 | Mn2+ | can partially replace Mg2+ in activation (in chloroplastic enzyme, not in cytoplasmic enzyme) | Euglena gracilis | |
6.1.1.9 | Ca2+ | no activity | Euglena gracilis | |
6.1.1.9 | Co2+ | can partially replace Mg2+ in reaction with chloroplastic enzyme only | Euglena gracilis | |
6.1.1.9 | KCl | 30 mM: enhances activity of the cytoplasmic enzyme, has no effect on the chloroplastic enzyme, above 30 mM: inhibits activity of cytoplasmic enzyme more strongly than that of chloroplastic enzyme | Euglena gracilis | |
6.1.1.9 | Mg2+ | divalent cation required, Mg2+ is most effective | Euglena gracilis | |
6.1.1.9 | Mg2+ | optimal Mg2+/ATP ratio is 2.5 for the chloroplastic and 1 for the cytoplasmic enzyme | Euglena gracilis | |
6.1.1.9 | Mn2+ | 60% of the activation with Mg2+ | Euglena gracilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.1.1.4 | Euglena gracilis | - |
- |
- |
6.1.1.9 | Euglena gracilis | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.4 | ATP + L-leucine + tRNALeu | chloroplastic enzyme: high specificity towards tRNAs, in contrast the cytoplasmic enzyme recognizes tRNAs from the bleached mutant and from yeast, but also some tRNALeu isoacceptors from E. coli | Euglena gracilis | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
6.1.1.9 | ATP + L-valine + tRNAVal | chloroplastic enzyme charges E. coli tRNA and not yeast tRNA, cytoplasmic enzyme is able to aminoacylate yeast tRNA but not E. coli tRNA | Euglena gracilis | AMP + diphosphate + L-valyl-tRNAVal | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.1.1.9 | 7.5 | 8 | Tris-HCl or cacodylate buffer | Euglena gracilis |