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Literature summary extracted from

  • Rivoal, J.; Plaxton, W.C.; Turpin, D.H.
    Purification and characterization of high- and low-molecular-mass isoforms of phosphoenolpyruvate carboxylase from Chlamydomonas reinhardtii. Kinetic structural and immunological evidence that the green algal enzyme is distinct from the prokaryotic and higher plant enzymes (1998), Biochem. J., 331, 201-209.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.31 dihydroxyacetone phosphate activates Chlamydomonas reinhardtii
4.1.1.31 glutamine activates Chlamydomonas reinhardtii

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.31 2-oxoglutarate
-
 Chlamydomonas reinhardtii
4.1.1.31 Asp
-
 Chlamydomonas reinhardtii
4.1.1.31 Glu
-
 Chlamydomonas reinhardtii
4.1.1.31 malate
-
 Chlamydomonas reinhardtii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.31 additional information
-
 additional information
-
 Chlamydomonas reinhardtii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.31 additional information
-
 sequencing of the N-terminus, peptide mapping and immunological data suggest that the catalytic subunit of the enzyme is not related to the prokaryotic enzyme and is only distantly related to higher plant C4 and C3 enzymes Chlamydomonas reinhardtii
4.1.1.31 100000
-
 4 * 100000, isoenzyme PEPC1, SDS-PAGE Chlamydomonas reinhardtii
4.1.1.31 400000
-
 low-molecular-mass isoenzyme PEPC1, gel filtration Chlamydomonas reinhardtii
4.1.1.31 650000
-
 high-molecular-weight isoenzyme PEPC2, gel filtration Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.31 Phosphoenolpyruvate + CO2 Chlamydomonas reinhardtii key enzyme in the supply of carbon skeleton for the assimilation of nitrogen by green algae ?
-
 ?
4.1.1.31 Phosphoenolpyruvate + CO2 Chlamydomonas reinhardtii CW-15 cc1883 key enzyme in the supply of carbon skeleton for the assimilation of nitrogen by green algae ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.31 Chlamydomonas reinhardtii
-
-
-
4.1.1.31 Chlamydomonas reinhardtii CW-15 cc1883
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.31 a high-molecular-mass isoform, PEPC2, and a low-molecular-mass isoform, PEPC1 Chlamydomonas reinhardtii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.31 18.11
-
-
 Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.31 phosphoenolpyruvate + CO2
-
 Chlamydomonas reinhardtii phosphate + oxaloacetate
-
 ?
4.1.1.31 phosphoenolpyruvate + CO2
-
 Chlamydomonas reinhardtii CW-15 cc1883 phosphate + oxaloacetate
-
 ?
4.1.1.31 Phosphoenolpyruvate + CO2 key enzyme in the supply of carbon skeleton for the assimilation of nitrogen by green algae Chlamydomonas reinhardtii ?
-
 ?
4.1.1.31 Phosphoenolpyruvate + CO2 key enzyme in the supply of carbon skeleton for the assimilation of nitrogen by green algae Chlamydomonas reinhardtii CW-15 cc1883 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.31 ? isoenzyme PEPC2 is a complex between the PEPC catalytic subunit of MW 100000 Da and other immunologically unrelated polypeptides of 50000-700000 Da Chlamydomonas reinhardtii
4.1.1.31 tetramer 4 * 100000, isoenzyme PEPC1, SDS-PAGE Chlamydomonas reinhardtii