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Literature summary extracted from

  • Sako, Y.; Takai, K.; Uchida, A.; Ishida, Y.
    Purification and characterization of phosphoenolpyruvate carboxylase from the hyperthermophilic archaeon Methanothermus sociabilis (1996), FEBS Lett., 392, 148-152.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.31 fructose 1,6-bisphosphate no effect Methanothermus sociabilis
4.1.1.31 glucose 6-phosphate no effect Methanothermus sociabilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.31 1.3
-
 phosphoenolpyruvate
-
 Methanothermus sociabilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.31 Mg2+
-
 Methanothermus sociabilis
4.1.1.31 Mn2+ activates Methanothermus sociabilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.31 60000
-
 4 * 60000, SDS-PAGE Methanothermus sociabilis
4.1.1.31 240000
-
 gel filtration Methanothermus sociabilis

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.31 Methanothermus sociabilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.31
-
 Methanothermus sociabilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.31 2.7
-
-
 Methanothermus sociabilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.31 phosphoenolpyruvate + CO2
-
 Methanothermus sociabilis phosphate + oxaloacetate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.31 tetramer 4 * 60000, SDS-PAGE Methanothermus sociabilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.31 85
-
-
 Methanothermus sociabilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.1.31 60 95 60°C: about 30% of maximal activity, 95°C: about 40% of maximal activity Methanothermus sociabilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.31 75
-
 2 h, no loss of activity Methanothermus sociabilis
4.1.1.31 80
-
 50% loss of activity after 240 min Methanothermus sociabilis
4.1.1.31 90
-
 50% loss of activity after 20 min Methanothermus sociabilis
4.1.1.31 100
-
 50% loss of activity after 1 min Methanothermus sociabilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.31 8.5
-
-
 Methanothermus sociabilis

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.31 acetyl-CoA no effect Methanothermus sociabilis