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Literature summary extracted from

  • Singal, H.R.; Singh, R.
    Purification and properties of phosphoenolpyruvate carboxylase from immature pods of chickpea (Cicer arietinum L.) (1986), Plant Physiol., 80, 369-373.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.31 3-Phosphoglyceric acid activates Cicer arietinum
4.1.1.31 alpha-glycerophosphate activates Cicer arietinum
4.1.1.31 fructose 1,6-bisphosphate activates Cicer arietinum
4.1.1.31 glucose 6-phosphate activates Cicer arietinum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.31 ATP
-
 Cicer arietinum
4.1.1.31 Ca2+ 5 mM, in presence of 5 mM Mg2+, 83% inhibition Cicer arietinum
4.1.1.31 CTP
-
 Cicer arietinum
4.1.1.31 GTP
-
 Cicer arietinum
4.1.1.31 malate
-
 Cicer arietinum
4.1.1.31 Mn2+ 5 mM, in presence of 5 mM Mg2+, 72% inhibition Cicer arietinum
4.1.1.31 oxaloacetate
-
 Cicer arietinum
4.1.1.31 UTP
-
 Cicer arietinum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.31 0.74
-
 phosphoenolpyruvate
-
 Cicer arietinum
4.1.1.31 7.6
-
 HCO3-
-
 Cicer arietinum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.31 Mg2+ required Cicer arietinum
4.1.1.31 Mg2+ Km: 0.56 mM Cicer arietinum
4.1.1.31 Mn2+ no activation Cicer arietinum
4.1.1.31 phosphate activates Cicer arietinum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.31 50000
-
 4 * 50000, SDS-PAGE Cicer arietinum
4.1.1.31 200000
-
 gel filtration Cicer arietinum

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.31 Cicer arietinum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.31
-
 Cicer arietinum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.31 pod immature Cicer arietinum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.31 0.137
-
-
 Cicer arietinum

Storage Stability

EC Number Storage Stability Organism
4.1.1.31 0-4°C, stable for 3 d Cicer arietinum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.31 phosphoenolpyruvate + CO2
-
 Cicer arietinum phosphate + oxaloacetate
-
 ?
4.1.1.31 phosphoenolpyruvate + HCO3-
-
 Cicer arietinum phosphate + oxaloacetate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.31 tetramer 4 * 50000, SDS-PAGE Cicer arietinum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.31 45
-
 2 min, 56% loss of activity Cicer arietinum
4.1.1.31 60
-
 2 min, complete loss of activity Cicer arietinum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.31 8.1
-
-
 Cicer arietinum