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Literature summary extracted from

  • Jetten, M.S.M.; Sinskey, A.J.
    Purification and properties of oxaloacetate decarboxylase from Corynebacterium glutamicum (1995), Antonie van Leeuwenhoek, 67, 221-227.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.1.1.112 synthesis optimization of oxaloacetate may increase lysine productivity of commercially used strains Corynebacterium glutamicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.112 ADP Ki: 1.2 mM Corynebacterium glutamicum
4.1.1.112 Avidin not Corynebacterium glutamicum
4.1.1.112 coenzyme A Ki: 2.4 mM Corynebacterium glutamicum
4.1.1.112 Cu2+ Ki: 0.2 mM Corynebacterium glutamicum
4.1.1.112 succinate
-
 Corynebacterium glutamicum
4.1.1.112 Zn2+ Ki: 0.8 mM Corynebacterium glutamicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.112 2.1
-
 oxaloacetate
-
 Corynebacterium glutamicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.112 Mn2+
-
 Micrococcus luteus
4.1.1.112 Mn2+ Km: 1.2 mM Corynebacterium glutamicum
4.1.1.112 Mn2+ required, can be substituted by Mg2+, Co2+, Ni2+, Ca2+ Corynebacterium glutamicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.112 31700
-
 4 * 31700, SDS-PAGE Corynebacterium glutamicum
4.1.1.112 118000
-
 gel filtration Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.112 oxaloacetate Corynebacterium glutamicum
-
 ?
-
 ?
4.1.1.112 oxaloacetate Micrococcus luteus
-
 ?
-
 ?
4.1.1.112 oxaloacetate Azotobacter vinelandii
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.112 Azotobacter vinelandii
-
-
-
4.1.1.112 Corynebacterium glutamicum
-
-
-
4.1.1.112 Micrococcus luteus
-
 formerly Micrococcus lysodeiktikus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.112
-
 Corynebacterium glutamicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.112 additional information
-
 strain-dependend Corynebacterium glutamicum
4.1.1.112 158
-
-
 Corynebacterium glutamicum

Storage Stability

EC Number Storage Stability Organism
4.1.1.112 -20°C, potassium phosphate buffer, pH 7, 50% glycerol, 2 month Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.112 Oxaloacetate
-
 Corynebacterium glutamicum Pyruvate + CO2
-
 ?
4.1.1.112 Oxaloacetate
-
 Micrococcus luteus Pyruvate + CO2
-
 ?
4.1.1.112 Oxaloacetate
-
 Azotobacter vinelandii Pyruvate + CO2
-
 ?
4.1.1.112 oxaloacetate
-
 Corynebacterium glutamicum ?
-
 ?
4.1.1.112 oxaloacetate
-
 Micrococcus luteus ?
-
 ?
4.1.1.112 oxaloacetate
-
 Azotobacter vinelandii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.112 tetramer 4 * 31700, SDS-PAGE Corynebacterium glutamicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.112 7 7.5
-
 Corynebacterium glutamicum

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.112 biotin not Corynebacterium glutamicum
4.1.1.112 biotin not Micrococcus luteus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.1.112 0.2
-
 Cu2+
-
 Corynebacterium glutamicum
4.1.1.112 0.8
-
 Zn2+
-
 Corynebacterium glutamicum
4.1.1.112 2.4
-
 coenzyme A
-
 Corynebacterium glutamicum