Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Clark, W.C.; Pass, P.S.; Venkataraman, B.; Hodgetts, R.B.
    Dopa decarboxylase from Drosophila melanogaster. Purification, characterization and an analysis of mutants (1978), Mol. Gen. Genet., 162, 287-297.
No PubMed abstract available

General Stability

EC Number General Stability Organism
4.1.1.28 three cycles of freezing, -45°C, and thawing have no effect on enzyme activity, in 0.10 M sodium phosphate buffer, pH 7.2, containg 1 mM dithiothreitol and 10% glycerol Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.28 Cu2+
-
 Drosophila melanogaster
4.1.1.28 Hg2+
-
 Drosophila melanogaster
4.1.1.28 N-acetyldopamine
-
 Drosophila melanogaster
4.1.1.28 NEM 1 mM, complete inhibition Drosophila melanogaster
4.1.1.28 Zn2+
-
 Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.28 additional information
-
 additional information
-
 Drosophila melanogaster
4.1.1.28 3.3
-
 L-Tyr
-
 Drosophila melanogaster

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.28 54000
-
 2 * 54000, SDS-PAGE Drosophila melanogaster
4.1.1.28 102000
-
 variable porosity acrylamide gel electrophoresis Drosophila melanogaster
4.1.1.28 113000
-
 sucrose density gradient sedimentation Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.28 Drosophila melanogaster
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.28
-
 Drosophila melanogaster

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.28 larva mature Drosophila melanogaster
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.28 4.07
-
-
 Drosophila melanogaster

Storage Stability

EC Number Storage Stability Organism
4.1.1.28 0°C, 0.05-0.01 M sodium phosphate buffer, pH 7.2, 1 mM dithiothreitol, stable for 6 weeks Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.28 3,4-Dihydroxyphenylalanine
-
 Drosophila melanogaster 3-Hydroxytyramine + CO2
-
 ?
4.1.1.28 Tyr
-
 Drosophila melanogaster ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.28 dimer 2 * 54000, SDS-PAGE Drosophila melanogaster

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.28 pyridoxal 5'-phosphate 0.0032-0.32 mM, enhances activity greater than 2fold Drosophila melanogaster