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Literature summary extracted from

  • Mehta, A.; Datta, A.
    Oxalate decarboxylase from Collybia velutipes. Purification, characterization, and cDNA cloning (1991), J. Biol. Chem., 266, 23548-23553.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.2
-
 Flammulina velutipes

General Stability

EC Number General Stability Organism
4.1.1.2 retains 45% of its activity after incubation with 10% SDS for 30 min at room temperature. Almost complete loss of activity after 30 min, in presence of 10% SDS Flammulina velutipes

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.2 phosphate competitive Flammulina velutipes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.2 4.5
-
 oxalate
-
 Flammulina velutipes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.2 55000
-
 x * 55000, enzyme decglycosylated by endo-beta-N-acetylglucosaminidase, SDS-PAGE Flammulina velutipes
4.1.1.2 64000
-
 x * 64000, glycosylated enzyme, SDS-PAGE Flammulina velutipes
4.1.1.2 560000
-
 the high apparent MW obtained by gel filtration can be due to the tendency of certain glycoproteins to interact noncovalently in solution, gel filtration Flammulina velutipes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.2 Oxalate Flammulina velutipes induction by oxalate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.2 Flammulina velutipes
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.1.1.2 glycoprotein 15% neutral sugar content Flammulina velutipes

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.2
-
 Flammulina velutipes

Storage Stability

EC Number Storage Stability Organism
4.1.1.2 4°C, 1 mg/ml protein, 0.02 M potassium acetate, more than 70% of the initial activity is retained after 4 months Flammulina velutipes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.2 Oxalate
-
 Flammulina velutipes Formate + CO2
-
 ?
4.1.1.2 Oxalate induction by oxalate Flammulina velutipes ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.2 ? x * 55000, enzyme decglycosylated by endo-beta-N-acetylglucosaminidase, SDS-PAGE Flammulina velutipes
4.1.1.2 ? x * 64000, glycosylated enzyme, SDS-PAGE Flammulina velutipes

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.2 30
-
 30 min, in presence of 10% SDS, retains 45% of its activity Flammulina velutipes
4.1.1.2 60
-
 30 min, in presence of 10% SDS, complete inactivation Flammulina velutipes
4.1.1.2 80
-
 20 min, 22% loss of activity Flammulina velutipes
4.1.1.2 96
-
 10 min, complete inactivation Flammulina velutipes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.2 3
-
-
 Flammulina velutipes