Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Edwards, R.
    Characterization of glutathione transferases and glutathione peroxidases in pea (Pisum sativum) (1996), Physiol. Plant., 98, 594-604.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.025
-
 fluorodifen
-
 Pisum sativum
2.5.1.18 0.55
-
 glutathione
-
 Pisum sativum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.18 CuCl2 0.05 mM and 0.5 mM increases activity of GPOX Pisum sativum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.18 27500
-
 1 * 30000 + 1 * 27500 or 1 * 30000 + 1 * 29000, SDS-PAGE, GSTF Pisum sativum
2.5.1.18 27500
-
 1 * 27500 + 1 * 29000, SDS-PAGE, GSTC and GPOX Pisum sativum
2.5.1.18 29000
-
 1 * 30000 + 1 * 27500 or 1 * 30000 + 1 * 29000, SDS-PAGE, GSTF Pisum sativum
2.5.1.18 29000
-
 1 * 27500 + 1 * 29000, SDS-PAGE, GSTC and GPOX Pisum sativum
2.5.1.18 30000
-
 1 * 30000 + 1 * 27500 or 1 * 30000 + 1 * 29000, SDS-PAGE, GSTF Pisum sativum
2.5.1.18 48000 54000 gel filtration Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.9 glutathione + ROOH Pisum sativum
-
 glutathione disulfide + ROH + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.9 Pisum sativum
-
-
-
2.5.1.18 Medicago sativa
-
 alfalfa
-
2.5.1.18 Phaseolus coccineus
-
 runner bean
-
2.5.1.18 Phaseolus vulgaris
-
 french bean
-
2.5.1.18 Pisum sativum
-
 pea
-
2.5.1.18 Trifolium repens
-
 white clover
-
2.5.1.18 Triticum aestivum
-
-
-
2.5.1.18 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.9
-
 Pisum sativum
2.5.1.18 GSTC: activity towards 1-chloro-2,4-dinitrobenzene, GSTF: activity towards the herbicide fluorodifen, GPOX: peroxidase activity Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.9 epicotyl
-
 Pisum sativum
-
1.11.1.9 root
-
 Pisum sativum
-
1.11.1.9 seedling
-
 Pisum sativum
-
2.5.1.18 root
-
 Triticum aestivum
-
2.5.1.18 root
-
 Pisum sativum
-
2.5.1.18 seedling etiolated Pisum sativum
-

Storage Stability

EC Number Storage Stability Organism
2.5.1.18 4°C, 30% loss of activity after 16 h Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.9 glutathione + ROOH
-
 Pisum sativum glutathione disulfide + ROH + H2O
-
 ?
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Phaseolus vulgaris S-2,4-dinitrophenylglutathione + HCl
-
 ?
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Triticum aestivum S-2,4-dinitrophenylglutathione + HCl
-
 ?
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Pisum sativum S-2,4-dinitrophenylglutathione + HCl
-
 ?
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Zea mays S-2,4-dinitrophenylglutathione + HCl
-
 ?
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Medicago sativa S-2,4-dinitrophenylglutathione + HCl
-
 ?
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Trifolium repens S-2,4-dinitrophenylglutathione + HCl
-
 ?
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Phaseolus coccineus S-2,4-dinitrophenylglutathione + HCl
-
 ?
2.5.1.18 glutathione + cumene hydroperoxide
-
 Pisum sativum ?
-
 ?
2.5.1.18 glutathione + cumene hydroperoxide
-
 Medicago sativa ?
-
 ?
2.5.1.18 glutathione + cumene hydroperoxide
-
 Trifolium repens ?
-
 ?
2.5.1.18 glutathione + fluorodifen
-
 Phaseolus vulgaris 4-nitrophenol + 2-nitro-4-trifluoromethylphenol-glutathione
-
 ?
2.5.1.18 glutathione + fluorodifen
-
 Triticum aestivum 4-nitrophenol + 2-nitro-4-trifluoromethylphenol-glutathione
-
 ?
2.5.1.18 glutathione + fluorodifen
-
 Pisum sativum 4-nitrophenol + 2-nitro-4-trifluoromethylphenol-glutathione
-
 ?
2.5.1.18 glutathione + fluorodifen
-
 Zea mays 4-nitrophenol + 2-nitro-4-trifluoromethylphenol-glutathione
-
 ?
2.5.1.18 glutathione + fluorodifen
-
 Medicago sativa 4-nitrophenol + 2-nitro-4-trifluoromethylphenol-glutathione
-
 ?
2.5.1.18 glutathione + fluorodifen
-
 Trifolium repens 4-nitrophenol + 2-nitro-4-trifluoromethylphenol-glutathione
-
 ?
2.5.1.18 glutathione + fluorodifen
-
 Phaseolus coccineus 4-nitrophenol + 2-nitro-4-trifluoromethylphenol-glutathione
-
 ?
2.5.1.18 additional information GSTC: activity towards 1-chloro-2,4-dinitrobenzene, GSTF: activity towards the herbicide fluorodifen, GPOX: peroxidase activity, overview: xenobiotic substrates Pisum sativum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.18 dimer
-
 Triticum aestivum
2.5.1.18 dimer
-
 Zea mays
2.5.1.18 dimer 1 * 30000 + 1 * 27500 or 1 * 30000 + 1 * 29000, SDS-PAGE, GSTF Pisum sativum
2.5.1.18 dimer 1 * 27500 + 1 * 29000, SDS-PAGE, GSTC and GPOX Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.18 9.3 9.5 cleavage of fluorodifen Pisum sativum