BRENDA - Enzyme Database

Purification and properties of an amine dehydrogenase from Pseudomonas K95 grown on 1,12-diaminododecane (DAD)

Niimura, Y.; Omori, T.; Minoda, Y.; Agric. Biol. Chem. 50, 1445-1451 (1986)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.9.1
isoniazid
-
Pseudomonas sp.
1.4.9.1
N-ethylmaleimide
-
Pseudomonas sp.
1.4.9.1
Neocuproine
slight
Pseudomonas sp.
1.4.9.1
Quinine
slight
Pseudomonas sp.
1.4.9.1
Semicarbazide
-
Pseudomonas sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4.9.1
0.003
-
1,12-diaminododecane
-
Pseudomonas sp.
1.4.9.1
0.033
-
12-aminododecanoic acid
-
Pseudomonas sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.4.9.1
47000
-
1 * 47000, SDS-PAGE
Pseudomonas sp.
1.4.9.1
56000
-
gel filtration
Pseudomonas sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.4.9.1
Pseudomonas sp.
-
K95
-
1.4.9.1
Pseudomonas sp. K95
-
K95
-
Purification (Commentary)
EC Number
Commentary
Organism
1.4.9.1
-
Pseudomonas sp.
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.4.9.1
3.9
-
-
Pseudomonas sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.9.1
1,12-diaminododecane + acceptor + H2O
-
396572
Pseudomonas sp.
? + NH3 + reduced acceptor
-
-
-
?
1.4.9.1
1,8-diaminooctane + H2O + acceptor
-
396572
Pseudomonas sp.
?
-
-
-
?
1.4.9.1
1,8-diaminooctane + H2O + acceptor
-
396572
Pseudomonas sp. K95
?
-
-
-
?
1.4.9.1
12-aminododecane + acceptor + H2O
-
396572
Pseudomonas sp.
dodecanal + NH3 + reduced acceptor
-
-
-
?
1.4.9.1
additional information
broad specificity
396572
Pseudomonas sp.
?
-
-
-
-
1.4.9.1
additional information
nonspecific oxidizing both short and long primary monoamines and diamines, polyamines, L-noradrenaline, histamine, benzylamine and di-n-hexylamine
396572
Pseudomonas sp.
?
-
-
-
-
1.4.9.1
additional information
broad specificity
396572
Pseudomonas sp. K95
?
-
-
-
-
1.4.9.1
additional information
nonspecific oxidizing both short and long primary monoamines and diamines, polyamines, L-noradrenaline, histamine, benzylamine and di-n-hexylamine
396572
Pseudomonas sp. K95
?
-
-
-
-
1.4.9.1
n-hexylamine + acceptor + H2O
-
396572
Pseudomonas sp.
hexanal + NH3 + reduced acceptor
-
-
-
?
1.4.9.1
n-hexylamine + acceptor + H2O
-
396572
Pseudomonas sp. K95
hexanal + NH3 + reduced acceptor
-
-
-
?
1.4.9.1
n-nonylamine + acceptor + H2O
-
396572
Pseudomonas sp.
nonanal + NH3 + reduced acceptor
-
-
-
?
1.4.9.1
RCH2NH2 + acceptor + H2O
acceptor phenazine methosulfate
396572
Pseudomonas sp.
RCHO + NH3 + reduced acceptor
-
-
-
?
1.4.9.1
RCH2NH2 + acceptor + H2O
acceptor phenazine methosulfate
396572
Pseudomonas sp. K95
RCHO + NH3 + reduced acceptor
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.4.9.1
monomer
1 * 47000, SDS-PAGE
Pseudomonas sp.
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.4.9.1
40
-
1,12-diaminododecane
Pseudomonas sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.4.9.1
7
-
1,12-diaminododecane
Pseudomonas sp.
1.4.9.1
8
-
12-aminododecanoic acid
Pseudomonas sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.9.1
isoniazid
-
Pseudomonas sp.
1.4.9.1
N-ethylmaleimide
-
Pseudomonas sp.
1.4.9.1
Neocuproine
slight
Pseudomonas sp.
1.4.9.1
Quinine
slight
Pseudomonas sp.
1.4.9.1
Semicarbazide
-
Pseudomonas sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4.9.1
0.003
-
1,12-diaminododecane
-
Pseudomonas sp.
1.4.9.1
0.033
-
12-aminododecanoic acid
-
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.4.9.1
47000
-
1 * 47000, SDS-PAGE
Pseudomonas sp.
1.4.9.1
56000
-
gel filtration
Pseudomonas sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.4.9.1
-
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.4.9.1
3.9
-
-
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.9.1
1,12-diaminododecane + acceptor + H2O
-
396572
Pseudomonas sp.
? + NH3 + reduced acceptor
-
-
-
?
1.4.9.1
1,8-diaminooctane + H2O + acceptor
-
396572
Pseudomonas sp.
?
-
-
-
?
1.4.9.1
1,8-diaminooctane + H2O + acceptor
-
396572
Pseudomonas sp. K95
?
-
-
-
?
1.4.9.1
12-aminododecane + acceptor + H2O
-
396572
Pseudomonas sp.
dodecanal + NH3 + reduced acceptor
-
-
-
?
1.4.9.1
additional information
broad specificity
396572
Pseudomonas sp.
?
-
-
-
-
1.4.9.1
additional information
nonspecific oxidizing both short and long primary monoamines and diamines, polyamines, L-noradrenaline, histamine, benzylamine and di-n-hexylamine
396572
Pseudomonas sp.
?
-
-
-
-
1.4.9.1
additional information
broad specificity
396572
Pseudomonas sp. K95
?
-
-
-
-
1.4.9.1
additional information
nonspecific oxidizing both short and long primary monoamines and diamines, polyamines, L-noradrenaline, histamine, benzylamine and di-n-hexylamine
396572
Pseudomonas sp. K95
?
-
-
-
-
1.4.9.1
n-hexylamine + acceptor + H2O
-
396572
Pseudomonas sp.
hexanal + NH3 + reduced acceptor
-
-
-
?
1.4.9.1
n-hexylamine + acceptor + H2O
-
396572
Pseudomonas sp. K95
hexanal + NH3 + reduced acceptor
-
-
-
?
1.4.9.1
n-nonylamine + acceptor + H2O
-
396572
Pseudomonas sp.
nonanal + NH3 + reduced acceptor
-
-
-
?
1.4.9.1
RCH2NH2 + acceptor + H2O
acceptor phenazine methosulfate
396572
Pseudomonas sp.
RCHO + NH3 + reduced acceptor
-
-
-
?
1.4.9.1
RCH2NH2 + acceptor + H2O
acceptor phenazine methosulfate
396572
Pseudomonas sp. K95
RCHO + NH3 + reduced acceptor
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.4.9.1
monomer
1 * 47000, SDS-PAGE
Pseudomonas sp.
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.4.9.1
40
-
1,12-diaminododecane
Pseudomonas sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.4.9.1
7
-
1,12-diaminododecane
Pseudomonas sp.
1.4.9.1
8
-
12-aminododecanoic acid
Pseudomonas sp.