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Literature summary extracted from

  • Goncalves-Butruille, M.; Szajner, P.; Torigoi, E.; Leite, A.; Arruda, P.
    Purification and Characterization of the Bifunctional Enzyme Lysine-Ketoglutarate Reductase-Saccharopine Dehydrogenase from Maize (1996), Plant Physiol., 110, 765-771.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.9 125000
-
 2 * 125000, SDS-PAGE, lysine-ketoglutarate reductase (EC 1. 5.1.8) and saccharopine dehydrogenase activity (EC 1.5.1.9) occur on a single protein Zea mays
1.5.1.9 260000
-
 gel filtration with Superdex, lysine-ketoglutarate reductase (EC 1. 5.1.8) and saccharopine dehydrogenase activity (EC 1.5.1.9) occur on a single protein Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.9 N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O Zea mays involved in lysine catabolism L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.9 Zea mays
-
 lysine-ketoglutarate reductase (EC 1.5.1.8) and saccharopine dehydrogenase activity (EC 1.5.1.9) occur on a single protein, called aminoadipic semialdehyde synthase
-
1.5.1.9 Zea mays
-
 maize hybrid F-352
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.9 lysine-ketoglutarate reductase (EC 1. 5.1.8) and saccharopine dehydrogenase activity (EC 1.5.1.9) occur on a single protein Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.9 endosperm
-
 Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.9 0.0024
-
 crude extract from immature endosperm Zea mays
1.5.1.9 2.14
-
 after purification Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.9 N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O
-
 Zea mays L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+
-
 r
1.5.1.9 N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O involved in lysine catabolism Zea mays L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.5.1.9 dimer 2 * 125000, SDS-PAGE, lysine-ketoglutarate reductase (EC 1. 5.1.8) and saccharopine dehydrogenase activity (EC 1.5.1.9) occur on a single protein Zea mays

Synonyms

EC Number Synonyms Comment Organism
1.5.1.9 SDH
-
 Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.9 9.5
-
-
 Zea mays

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.9 6.5 10 no activity below pH 6 and over pH 10 Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.9 NAD+
-
 Zea mays
1.5.1.9 NADP+ 9% of the rate with NAD+ Zea mays