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Literature summary extracted from

  • Nozaki, M.
    Nonheme iron dioxygenase (1974), Mol. Mech. Oxygen Activ. (Hayaishi, O., ed.) Academic Press, New York, , 135-165.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.5 ascorbate required for maximal activity at pH 6 Pseudomonas fluorescens
1.13.11.5 ascorbate required for maximal activity at pH 7 Bos taurus
1.13.11.5 glutathione required for maximal activity at pH 6 and 5.4 Pseudomonas fluorescens
1.13.11.5 glutathione reduced glutathione is required for maximal activity at pH 7 Bos taurus
1.13.11.5 additional information pH 7.0: a combination of ascorbate, reduced glutathione and ferrous iron is required for activation, pH 5.3: ferrous iron alone fully activates Bos taurus
1.13.11.19 hydroxylamine cofactor-like compound Equus caballus
1.13.11.19 hydroxylamine sulfide, elemental sulfur, elemental selenium or hydroxylamine required in catalytic amount, inhibition when added over a critical concentration (with the exception of hydroxylamine) Equus caballus
1.13.11.19 Se cofactor-like compound Equus caballus
1.13.11.19 Se sulfide, elemental sulfur, elemental selenium or hydroxylamine required in catalytic amount, inhibition when added over a critical concentration (with the exception of hydroxylamine) Equus caballus
1.13.11.19 Sulfide cofactor-like compound Equus caballus
1.13.11.19 Sulfide sulfide, elemental sulfur, elemental selenium or hydroxylamine required in catalytic amount, inhibition when added over a critical concentration (with the exception of hydroxylamine) Equus caballus
1.13.11.19 sulfur cofactor-like compound Equus caballus
1.13.11.19 sulfur sulfide, elemental sulfur, elemental selenium or hydroxylamine required in catalytic amount, inhibition when added over a critical concentration (with the exception of hydroxylamine) Equus caballus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.11.5
-
 Pseudomonas fluorescens
1.13.11.9
-
 Pseudomonas putida
1.13.11.15
-
 Pseudomonas putida

General Stability

EC Number General Stability Organism
1.13.11.5 10% acetone stabilizes Bos taurus
1.13.11.5 unlike the mammalian enzyme, the bacterial enzyme is fairly stable on aging or during storage Pseudomonas fluorescens
1.13.11.9 combination of dithiothreitol and FeSO4 is detrimental during long-term incubation at 0°C Pseudomonas putida
1.13.11.9 dithiothreitol stabilizes Pseudomonas putida
1.13.11.15 stabilization in presence of low concentrations of acetone or ethanol Pseudomonas putida
1.13.11.25 acetone stabilizes Rhodococcus equi

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.5 Lewisite inhibits by competing with Fe2+ for a common enzymic binding site Bos taurus
1.13.11.5 Methylmercuric bromide inhibits by competing with Fe2+ for a common enzymic binding site Bos taurus
1.13.11.5 p-chloromercuribenzoate inhibits by competing with Fe2+ for a common enzymic binding site Bos taurus
1.13.11.5 p-chloromercuribenzoate competitive inhibition with respect to Fe2+ but not to homogentisate Pseudomonas fluorescens
1.13.11.9 1,10-phenanthroline
-
 Pseudomonas putida
1.13.11.9 2,2'-dipyridyl
-
 Pseudomonas putida
1.13.11.9 EDTA
-
 Pseudomonas putida
1.13.11.9 H2O2
-
 Pseudomonas putida
1.13.11.9 KCN
-
 Pseudomonas putida
1.13.11.9 N-ethylmaleimide
-
 Pseudomonas putida
1.13.11.9 p-chloromercuribenzoate
-
 Pseudomonas putida
1.13.11.19 S sulfide, elemental sulfur, elemental selenium or hydroxylamine required in catalytic amount, inhibition when added over a critical concentration (with the exception of hydroxylamine) Equus caballus
1.13.11.19 Se sulfide, elemental sulfur, elemental selenium or hydroxylamine required in catalytic amount, inhibition when added over a critical concentration (with the exception of hydroxylamine) Equus caballus
1.13.11.19 Sulfide sulfide, elemental sulfur, elemental selenium or hydroxylamine required in catalytic amount, inhibition when added over a critical concentration (with the exception of hydroxylamine) Equus caballus
1.13.11.25 2,2'-dipyridyl noncompetitive inhibition Rhodococcus equi
1.13.11.25 4-hydroxymercuribenzoate 1 mM, 87% inhibition after 10 min Rhodococcus equi
1.13.11.25 4-Isopropylcatechol competitive vs. organic substrate, uncompetitive vs. O2 Rhodococcus equi
1.13.11.25 8-hydroxyquinoline 1 mM, 90% inhibition after 10 min, noncompetitive inhibition Rhodococcus equi
1.13.11.25 o-phenanthroline 1 mM, 68% inhibition after 10 min, noncompetitive inhibition Rhodococcus equi
1.13.11.25 sodium iodoacetate 1 mM, 70% inhibition after 10 min Rhodococcus equi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.5 0.6
-
 homogentisate at pH 6 Pseudomonas fluorescens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.11.5 soluble
-
 Bos taurus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.5 Fe2+ ferrous mercaptans at the active center Bos taurus
1.13.11.5 Fe2+ Fe2+ is required as cofactor Pseudomonas fluorescens
1.13.11.5 Fe2+ ferrous ion is required for maximal activity at pH 7 and pH 5.3 Bos taurus
1.13.11.15 Iron 4-5 gatom of iron per mol of enzyme, most of the iron is involved in the association of the subunits, at least 1 gatom of iron is at the active site Pseudomonas putida
1.13.11.19 Fe a non-heme-iron protein, contains 1 g-atom of iron per mol of enzyme (based on a MW of 83000), nearly all of the iron is in the ferric state Equus caballus
1.13.11.25 Fe2+ 1.13 gatom per mol of enzyme, stimulation of reaction Rhodococcus equi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.5 380000
-
 ultracentrifugal analysis Pseudomonas fluorescens
1.13.11.9 39500
-
 x * 39500, SDS-PAGE Pseudomonas putida
1.13.11.9 242000
-
 sucrose density gradient centrifugation (+ dithiothreitol) Pseudomonas putida
1.13.11.19 50000
-
 2 * 50000 SDS-PAGE Equus caballus
1.13.11.19 100000
-
 method not mentioned Equus caballus
1.13.11.25 286000
-
-
 Rhodococcus equi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.25 3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + O2 Rhodococcus equi
-
 3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.5 Bos taurus
-
-
-
1.13.11.5 Pseudomonas fluorescens
-
-
-
1.13.11.9 Pseudomonas putida
-
-
-
1.13.11.15 Pseudomonas putida
-
-
-
1.13.11.19 Equus caballus
-
-
-
1.13.11.25 Rhodococcus equi
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.13.11.5 Fe2+, organic mercurials and reducing agents protect the enzyme from irreversible aerobic oxidation Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.5 partial purification Bos taurus
1.13.11.15
-
 Pseudomonas putida
1.13.11.19
-
 Equus caballus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.13.11.25 3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + O2 = 3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate proximal extradiol cleavage Rhodococcus equi
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.19 kidney
-
 Equus caballus
-

Storage Stability

EC Number Storage Stability Organism
1.13.11.19 0°C, 70% saturated ammonium sulfate, stable for months Equus caballus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.5 homogentisate + O2
-
 Pseudomonas fluorescens 4-maleylacetoacetate
-
 ?
1.13.11.9 2,5-dihydroxypyridine + O2 + H2O
-
 Pseudomonas putida maleamate + formate
-
 ?
1.13.11.15 3,4-dihydroxyphenylacetate + O2
-
 Pseudomonas putida 2-hydroxy-5-carboxymethylmuconate semialdehyde
-
 ?
1.13.11.19 cysteamine + O2
-
 Equus caballus hypotaurine
-
 ?
1.13.11.25 3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + O2
-
 Rhodococcus equi 3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate
-
 ?
1.13.11.25 3-isopropylcatechol + O2
-
 Rhodococcus equi ?
-
 ?
1.13.11.25 3-tert-butyl-5-methylcatechol + O2
-
 Rhodococcus equi ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.9 ? x * 39500, SDS-PAGE Pseudomonas putida
1.13.11.19 dimer 2 * 50000 SDS-PAGE Equus caballus