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Literature summary extracted from

  • Scheer, H.; Robaire, B.
    Solubilization and partial characterization of rat epididymal delta 4-steroid 5alpha-reductase (cholestenone 5alpha-reductase) (1983), Biochem. J., 211, 65-74.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.22 17alpha-hydroxyprogesterone
-
 Rattus norvegicus
1.3.1.22 androst-4-enedione
-
 Rattus norvegicus
1.3.1.22 NADP+
-
 Rattus norvegicus
1.3.1.22 progesterone competitive Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.22 0.0002
-
 testosterone nuclear and microsomal solubilized enzyme Rattus norvegicus
1.3.1.22 0.00102
-
 NADPH nuclear enzyme Rattus norvegicus
1.3.1.22 0.0376
-
 NADPH microsomal membrane-bound enzyme Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.22 microsome
-
 Rattus norvegicus
-
-
1.3.1.22 nucleus
-
 Rattus norvegicus 5634
-

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.22 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.22 epididymis
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
1.3.1.22 -20°C, 1 week, solubilized enzyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.22 cholest-4-en-3-one + NADPH
-
 Rattus norvegicus 5-alpha-cholestan-3-one + NADP+
-
 ?
1.3.1.22 testosterone + NADPH + H+
-
 Rattus norvegicus 17beta-hydroxy-5alpha-androstan-3-one + NADP+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.22 5.8 7.5
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.1.22 5 8.5 at pH 5 and 8.5: about 50% of activity maximum Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.22 NADPH
-
 Rattus norvegicus