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Literature summary extracted from
- Extraction of the reduced nicotinamide adenine dinucleotide phosphate:delta 4-3-ketosteroid-5-alpha oxidoreductase of rat prostate with digitonin and potassium chloride (1974), Biochemistry, 13, 450-456.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.3.1.22 | glycerol stabilizes | Rattus norvegicus |
| 1.3.1.22 | NADPH stabilizes | Rattus norvegicus |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.3.1.22 | endoplasmic reticulum | - |
Rattus norvegicus | 5783 | - |
| 1.3.1.22 | membrane | of endoplasmic reticulum | Rattus norvegicus | 16020 | - |
| 1.3.1.22 | nuclear envelope | - |
Rattus norvegicus | 5635 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.3.1.22 | 25000 | 35000 | gel filtration | Rattus norvegicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.3.1.22 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.3.1.22 | partial | Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.3.1.22 | prostate gland | ventral | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.3.1.22 | 1090 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.1.22 | cholest-4-en-3-one + NADPH | - |
Rattus norvegicus | 5-alpha-cholestan-3-one + NADP+ | - |
? |  |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.3.1.22 | 6.9 | - |
- |
Rattus norvegicus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.3.1.22 | 5.7 | 8.4 | in the cold | Rattus norvegicus |
| 1.3.1.22 | 6.6 | 7.8 | 4 days, in the cold, presence of KCl and NADH | Rattus norvegicus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.1.22 | NADPH | - |
Rattus norvegicus | |
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Release 2023.1 (January 2023)
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