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Literature summary extracted from

  • Wolfe, M.D.; Altier, D.J.; Stubna, A.; Popescu, C.V.; Muenck, E.; Lipscomb, J.D.
    Benzoate 1,2-dioxygenase from Pseudomonas putida: single turnover kinetics and regulation of a two-component Rieske dioxygenase (2002), Biochemistry, 41, 9611-9626.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.12.10 Iron oxygenase component: 2.7 mol/mol of alphabeta subunit, each alpha subunit contains a Rieske [2Fe-2S] cluster and a mononuclear iron site, reductase component: 2.02 mol/monomer Pseudomonas putida
1.14.12.10 Iron each alpha-subunit contains a Rieske [2Fe-2S] cluster and a mononuclear iron site Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.12.10 19000
-
 alpha3,beta3, 3 * 19000 + 3 * 49000, oxygenase component, SDS-PAGE Pseudomonas putida
1.14.12.10 38000
-
 reductase component Pseudomonas putida
1.14.12.10 49000
-
 alpha3,beta3, 3 * 19000 + 3 * 49000, oxygenase component, SDS-PAGE Pseudomonas putida
1.14.12.10 195000
-
 sedimentation analysis Pseudomonas putida
1.14.12.10 195000
-
 oxygenase component, sedimentation analysis Pseudomonas putida
1.14.12.10 204000
-
 SDS-PAGE Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.10 Pseudomonas putida
-
-
-
1.14.12.10 Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.12.10 benzoate oxygenase and reductase component Pseudomonas putida
1.14.12.10 DEAE-Sepharose Fast Flow column chromatography and Ultrogel AcA 34 gel filtration Pseudomonas putida

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.12.10 benzoate + NADH + H+ + O2 = (1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+ proposed catalytic cycle Pseudomonas putida
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.12.10 1.36
-
 benzoate oxygenase Pseudomonas putida
1.14.12.10 790
-
 benzoate reductase, reduction of K3Fe(CN)6 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.10 3-chlorobenzoate + O2 + NADH + H+
-
 Pseudomonas putida 3-chloro-2-hydro-1,2-dihydroxybenzoate + NAD+
-
 ?
1.14.12.10 3-chlorobenzoate + O2 + NADH + H+
-
 Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 3-chloro-2-hydro-1,2-dihydroxybenzoate + NAD+
-
 ?
1.14.12.10 3-fluorobenzoate + O2 + NADH + H+
-
 Pseudomonas putida 3-fluoro-2-hydro-1,2-dihydroxybenzoate + NAD+
-
 ?
1.14.12.10 3-fluorobenzoate + O2 + NADH + H+
-
 Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 3-fluoro-2-hydro-1,2-dihydroxybenzoate + NAD+
-
 ?
1.14.12.10 3-methylbenzoate + O2 + NADH + H+
-
 Pseudomonas putida 3-methyl-2-hydro-1,2-dihydroxybenzoate + NAD+
-
 ?
1.14.12.10 3-methylbenzoate + O2 + NADH + H+
-
 Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 3-methyl-2-hydro-1,2-dihydroxybenzoate + NAD+
-
 ?
1.14.12.10 benzoate + O2 + NADH
-
 Pseudomonas putida 2-hydro-1,2-dihydroxybenzoate + NAD+
-
 ?
1.14.12.10 benzoate + O2 + NADH
-
 Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 2-hydro-1,2-dihydroxybenzoate + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.12.10 hexamer alpha3,beta3, 3 * 19000 + 3 * 49000, oxygenase component, SDS-PAGE Pseudomonas putida
1.14.12.10 monomer 1 * 38000, reductase component, sedimentation analysis, SDS-PAGE Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.14.12.10 BZDO
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.10 FAD 1 FAD per enzyme monomer Pseudomonas putida
1.14.12.10 iron-sulfur center each alpha-subunit contains a Rieske [2Fe-2S] cluster and a mononuclear iron site Pseudomonas putida
1.14.12.10 NADH
-
 Pseudomonas putida