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Literature summary extracted from
- Subunit structure of oxygenase component in benzoate-1,2-dioxygenase system from Pseudomonas arvilla C-1 (1982), J. Biol. Chem., 257, 12497-12502.
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.12.10 | Iron | iron sulfur protein with [2Fe-2S]clusters and additional iron atoms | Pseudomonas putida |  |
| 1.14.12.10 | Iron | alpha-subunit contains 8.2 mol of iron | Pseudomonas putida | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.12.10 | 20000 | - |
alpha3,beta3, 3 * 20000 + 3 * 50000, oxygenase component of the enzyme system, SDS-PAGE | Pseudomonas putida |
| 1.14.12.10 | 38000 | - |
1 * 38000, reductase component of the enzyme system | Pseudomonas putida |
| 1.14.12.10 | 50000 | - |
alpha3,beta3, 3 * 20000 + 3 * 50000, oxygenase component of the enzyme system, SDS-PAGE | Pseudomonas putida |
| 1.14.12.10 | 201000 | - |
sedimentation equilibrium | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.12.10 | benzoate + O2 + NADH + H+ | Pseudomonas putida | - |
2-hydro-1,2-dihydroxybenzoate + NAD+ | - |
? | |
| 1.14.12.10 | benzoate + O2 + NADH + H+ | Pseudomonas putida C-1 | - |
2-hydro-1,2-dihydroxybenzoate + NAD+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.12.10 | Pseudomonas putida | - |
C-1 | - |
| 1.14.12.10 | Pseudomonas putida C-1 | - |
C-1 | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.12.10 | benzoate + O2 + NADH + H+ | - |
Pseudomonas putida | 2-hydro-1,2-dihydroxybenzoate + NAD+ | - |
? | |
| 1.14.12.10 | benzoate + O2 + NADH + H+ | - |
Pseudomonas putida C-1 | 2-hydro-1,2-dihydroxybenzoate + NAD+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.12.10 | hexamer | alpha3,beta3, 3 * 20000 + 3 * 50000, oxygenase component of the enzyme system, SDS-PAGE | Pseudomonas putida |
| 1.14.12.10 | monomer | 1 * 38000, reductase component of the enzyme system | Pseudomonas putida |
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