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Literature summary extracted from

  • Yamanaka, T.; Fujii, K.
    Cytochrome a-type terminal oxidase derived from Thiobacillus novellus. Molecular and enzymatic properties (1980), Biochim. Biophys. Acta, 591, 53-62.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.1.9 azide 0.014 mM, 50% inhibition of tuna cytochrome c oxidation Starkeya novella
7.1.1.9 CN- 0.004 mM, 50% inhibition of tuna cytochrome c oxidation Starkeya novella
7.1.1.9 phosphate not with yeast cytochrome c Starkeya novella

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1.1.9 0.011
-
ferrocytochrome c
-
Starkeya novella

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.1.1.9 23000
-
1 * 23000 + 1 * 32000, SDS-PAGE Starkeya novella
7.1.1.9 23000
-
x * 32000 + x * 23000, SDS-PAGE Starkeya novella
7.1.1.9 32000
-
1 * 23000 + 1 * 32000, SDS-PAGE Starkeya novella
7.1.1.9 32000
-
x * 32000 + x * 23000, SDS-PAGE Starkeya novella

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.9 Starkeya novella
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.1.9 Triton X-100, ammonium sulfate, DEAE-cellulose, Sephadex G-150 Starkeya novella

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.1.1.9 10.4
-
-
Starkeya novella

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.9 ferrocytochrome c + O2 Saccharomyces cerevisiae cytochrome c Starkeya novella ferricytochrome c + H2O
-
?
7.1.1.9 ferrocytochrome c + O2 horse ferrocytochrome c Starkeya novella ferricytochrome c + H2O
-
?
7.1.1.9 ferrocytochrome c + O2 cow cytochrome c Starkeya novella ferricytochrome c + H2O
-
?
7.1.1.9 ferrocytochrome c + O2 tuna cytochrome c Starkeya novella ferricytochrome c + H2O
-
?
7.1.1.9 ferrocytochrome c + O2 + H+
-
Starkeya novella ferricytochrome c + H2O
-
r

Subunits

EC Number Subunits Comment Organism
7.1.1.9 ? x * 32000 + x * 23000, SDS-PAGE Starkeya novella
7.1.1.9 dimer 1 * 23000 + 1 * 32000, SDS-PAGE Starkeya novella

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.1.1.9 additional information
-
additional information above 20 mM phosphate: rapid decrease of kcat with Candida krusei, tuna, Thiobacillus novellus, and horse cytochrome c Starkeya novella
7.1.1.9 20
-
ferrocytochrome c Thiobacillus novellus cytochrome c, 12.5 mM phosphate at pH 5.5 and 20°C Starkeya novella
7.1.1.9 45.8
-
ferrocytochrome c Candida krusei cytochrome c, 30 mM phosphate at pH 5.5 and 20°C Starkeya novella

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1.1.9 5.5
-
phosphate buffer Starkeya novella