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Literature summary extracted from

  • Yamazaki, T.; Fukumori, Y.; Yamanaka, T.
    Cytochrome a1 of Nitrosomonas europaea resemble aa3-type cytochrome c oxidase in many respects (1985), Biochim. Biophys. Acta, 810, 174-183.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.1.1.9 cardiolipin
-
 Nitrosomonas europaea

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.1.9 azide 1 mM, 50% inhibition of horse cytochrome c oxidation, 0.85 mM, 50% inhibition of horse cytochrome c oxidation in the presence of cardiolipin, 6.5 mM, 50% inhibition of Candida krusei cytochrome c oxidation, 1.5 mM, 50% inhibition of Nitrosomonas europaea cytochrome c oxidation Nitrosomonas europaea
7.1.1.9 CN- 0.13 mM, 50% inhibition of horse cytochrome c oxidation, 0.08 mM 50% inhibition of horse and Candida krusei cytochrome c oxidation in the presence of cardiolipin, 0.06 mM, 50% inhibition of Candida krusei cytochrome c oxidation, 0.05 mM, 50% inhibition of Nitrosomonas europaea cytochrome c oxidation Nitrosomonas europaea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1.1.9 0.0037
-
 ferrocytochrome c Candida krusei cytochrome c, in the presence of cardiolipin Nitrosomonas europaea
7.1.1.9 0.0038
-
 ferrocytochrome c horse cytochrome c, in the presence of cardiolipin Nitrosomonas europaea
7.1.1.9 0.0081
-
 ferrocytochrome c horse cytochrome c Nitrosomonas europaea
7.1.1.9 0.016
-
 ferrocytochrome c Candida krusei cytochrome c Nitrosomonas europaea
7.1.1.9 0.031
-
 ferrocytochrome c Nitrosomonas europaea cytochrome c Nitrosomonas europaea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.1.1.9 Cu
-
 Nitrosomonas europaea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.1.1.9 33000
-
 1 * 50000 + 1 * 33000, SDS-PAGE Nitrosomonas europaea
7.1.1.9 50000
-
 1 * 50000 + 1 * 33000, SDS-PAGE Nitrosomonas europaea
7.1.1.9 83000
-
 calculation from heme content Nitrosomonas europaea

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.9 Nitrosomonas europaea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.1.9 Triton X-100, DEAE-cellulose, Sephacryl S-300 Nitrosomonas europaea

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.1.1.9 0.0141
-
-
 Nitrosomonas europaea
7.1.1.9 0.258
-
 in the presence of cardiolipin Nitrosomonas europaea

Storage Stability

EC Number Storage Stability Organism
7.1.1.9 -20°C, 10 mM Tris-HCl buffer, pH 8.0, 0.5% Tween 20, several months, stable Nitrosomonas europaea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.9 ferrocytochrome c + O2 + H+
-
 Nitrosomonas europaea ferricytochrome c + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
7.1.1.9 dimer 1 * 50000 + 1 * 33000, SDS-PAGE Nitrosomonas europaea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.1.1.9 0.57
-
 ferrocytochrome c horse cytochrome c Nitrosomonas europaea
7.1.1.9 1.6
-
 ferrocytochrome c Candida krusei cytochrome c Nitrosomonas europaea
7.1.1.9 1.8
-
 ferrocytochrome c Nitrosomonaseuropaea cytochrome c Nitrosomonas europaea
7.1.1.9 14
-
 ferrocytochrome c Candida krusei cytochrome c, in the presence of cardiolipin Nitrosomonas europaea
7.1.1.9 32
-
 ferrocytochrome c horse cytochrome c, in the presence of cardiolipin Nitrosomonas europaea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1.1.9 5.6
-
 electron donor: cytochrome c from Nitrosomonas europaea Nitrosomonas europaea
7.1.1.9 6.1
-
 electron donor: cytochrome c from horse heart and Candida krusei Nitrosomonas europaea