Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Jeffrey, A.M.; Knight, M.; Evans, W.C.
    The bacterial degradation of flavonoids. Hydroxylation of the A-ring of taxifolin by a soil pseudomonad (1972), Biochem. J., 130, 373-381.
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.19 taxifolin + NADH + O2 Pseudomonas sp.
-
 2,3-dihydrogossypetin + NAD+ + H2O
-
 ?
1.14.13.19 taxifolin + NADPH + O2 Pseudomonas sp.
-
 2,3-dihydrogossypetin + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.19 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.19 partial Pseudomonas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.19 cell culture
-
 Pseudomonas sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.19 (-)-fustin + NADH + O2
-
 Pseudomonas sp. ? + NAD+ + H2O product not identified ?
1.14.13.19 taxifolin + NADH + O2
-
 Pseudomonas sp. 2,3-dihydrogossypetin + NAD+ + H2O
-
 ?
1.14.13.19 taxifolin + NADPH + O2
-
 Pseudomonas sp. 2,3-dihydrogossypetin + NADP+ + H2O
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.13.19 40
-
 25% loss of activity in 10 min Pseudomonas sp.
1.14.13.19 50
-
 95% loss of activity in 10 min Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.19 8
-
-
 Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.13.19 6
-
 20% of maximal activity Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.19 FAD
-
 Pseudomonas sp.
1.14.13.19 NADH twice oxidized compared to NADPH Pseudomonas sp.
1.14.13.19 NADPH
-
 Pseudomonas sp.