BRENDA - Enzyme Database

Purification, general properties and two other catalytic activities of alpha-ketoglutarate:glyoxylate carboligase of Mycobacterium phlei

Yamasaki, H.; Moriyama, T.; Biochim. Biophys. Acta 242, 637-647 (1971)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.2.1.5
EDTA
93% inhibition at 1 mM
Mycolicibacterium phlei
2.2.1.5
iodoacetic acid
83% inhibition at 3.3 mM
Mycolicibacterium phlei
2.2.1.5
p-chloromercuriphenylsulfonic acid
complete inhibition at 1 mM
Mycolicibacterium phlei
2.2.1.5
Zn2+
97% inhibition at 1 mM
Mycolicibacterium phlei
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.2.1.5
2
-
2-oxoglutarate
-
Mycolicibacterium phlei
2.2.1.5
3.2
-
glyoxylate
-
Mycolicibacterium phlei
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.2.1.5
Mg2+
31% activation at 1 mM
Mycolicibacterium phlei
2.2.1.5
Mn2+
175% activation at 1 mM
Mycolicibacterium phlei
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.2.1.5
Mycolicibacterium phlei
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.2.1.5
-
Mycolicibacterium phlei
Reaction
EC Number
Reaction
Commentary
Organism
2.2.1.5
2-oxoglutarate + glyoxylate = 2-hydroxy-3-oxoadipate + CO2
mechanism
Mycolicibacterium phlei
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.2.1.5
2.21
-
-
Mycolicibacterium phlei
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.2.1.5
2-oxoglutarate
-
395927
Mycolicibacterium phlei
? + CO2
-
395927
Mycolicibacterium phlei
-
2.2.1.5
2-oxoglutarate + acetaldehyde
-
395927
Mycolicibacterium phlei
5-hydroxy-4-oxohexanoate + CO2
-
395927
Mycolicibacterium phlei
-
2.2.1.5
2-oxoglutarate + glyoxylate
-
395927
Mycolicibacterium phlei
2-hydroxy-3-oxohexandioate + CO2
further spontaneous decarboxylation in the presence of acid
395927
Mycolicibacterium phlei
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.2.1.5
37
-
enzyme assay at
Mycolicibacterium phlei
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.2.1.5
6.3
-
-
Mycolicibacterium phlei
2.2.1.5
6.5
-
enzyme assay at
Mycolicibacterium phlei
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.2.1.5
thiamine diphosphate
-
Mycolicibacterium phlei
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.2.1.5
thiamine diphosphate
-
Mycolicibacterium phlei
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.2.1.5
EDTA
93% inhibition at 1 mM
Mycolicibacterium phlei
2.2.1.5
iodoacetic acid
83% inhibition at 3.3 mM
Mycolicibacterium phlei
2.2.1.5
p-chloromercuriphenylsulfonic acid
complete inhibition at 1 mM
Mycolicibacterium phlei
2.2.1.5
Zn2+
97% inhibition at 1 mM
Mycolicibacterium phlei
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.2.1.5
2
-
2-oxoglutarate
-
Mycolicibacterium phlei
2.2.1.5
3.2
-
glyoxylate
-
Mycolicibacterium phlei
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.2.1.5
Mg2+
31% activation at 1 mM
Mycolicibacterium phlei
2.2.1.5
Mn2+
175% activation at 1 mM
Mycolicibacterium phlei
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.2.1.5
-
Mycolicibacterium phlei
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.2.1.5
2.21
-
-
Mycolicibacterium phlei
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.2.1.5
2-oxoglutarate
-
395927
Mycolicibacterium phlei
? + CO2
-
395927
Mycolicibacterium phlei
-
2.2.1.5
2-oxoglutarate + acetaldehyde
-
395927
Mycolicibacterium phlei
5-hydroxy-4-oxohexanoate + CO2
-
395927
Mycolicibacterium phlei
-
2.2.1.5
2-oxoglutarate + glyoxylate
-
395927
Mycolicibacterium phlei
2-hydroxy-3-oxohexandioate + CO2
further spontaneous decarboxylation in the presence of acid
395927
Mycolicibacterium phlei
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.2.1.5
37
-
enzyme assay at
Mycolicibacterium phlei
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.2.1.5
6.3
-
-
Mycolicibacterium phlei
2.2.1.5
6.5
-
enzyme assay at
Mycolicibacterium phlei