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Literature summary extracted from

  • Yamasaki, H.; Moriyama, T.
    Purification, general properties and two other catalytic activities of alpha-ketoglutarate:glyoxylate carboligase of Mycobacterium phlei (1971), Biochim. Biophys. Acta, 242, 637-647.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.5 EDTA 93% inhibition at 1 mM Mycolicibacterium phlei
2.2.1.5 iodoacetic acid 83% inhibition at 3.3 mM Mycolicibacterium phlei
2.2.1.5 p-chloromercuriphenylsulfonic acid complete inhibition at 1 mM Mycolicibacterium phlei
2.2.1.5 Zn2+ 97% inhibition at 1 mM Mycolicibacterium phlei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.5 2
-
 2-oxoglutarate
-
 Mycolicibacterium phlei
2.2.1.5 3.2
-
 glyoxylate
-
 Mycolicibacterium phlei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.5 Mg2+ 31% activation at 1 mM Mycolicibacterium phlei
2.2.1.5 Mn2+ 175% activation at 1 mM Mycolicibacterium phlei

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.5 Mycolicibacterium phlei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.5
-
 Mycolicibacterium phlei

Reaction

EC Number Reaction Comment Organism Reaction ID
2.2.1.5 2-oxoglutarate + glyoxylate = 2-hydroxy-3-oxoadipate + CO2 mechanism Mycolicibacterium phlei
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2.1.5 2.21
-
-
 Mycolicibacterium phlei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.5 2-oxoglutarate
-
 Mycolicibacterium phlei ? + CO2
-
 ?
2.2.1.5 2-oxoglutarate + acetaldehyde
-
 Mycolicibacterium phlei 5-hydroxy-4-oxohexanoate + CO2
-
 ?
2.2.1.5 2-oxoglutarate + glyoxylate
-
 Mycolicibacterium phlei 2-hydroxy-3-oxohexandioate + CO2 further spontaneous decarboxylation in the presence of acid ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.2.1.5 37
-
 enzyme assay at Mycolicibacterium phlei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.5 6.3
-
-
 Mycolicibacterium phlei
2.2.1.5 6.5
-
 enzyme assay at Mycolicibacterium phlei

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.5 thiamine diphosphate
-
 Mycolicibacterium phlei